Recombinant Human sFRP-5 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human sFRP-5 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.2-2 μg/mL in the presence of 750 ng/mL Recombinant Human Wnt‑3a (Catalog # 5036-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived human sFRP-5 protein
Glu30-His317, with an N-terminal HA (YPYDVPDYA) tag
Accession #
N-terminal Sequence
Tyr (HA), Trp37 & Ser46
Protein/Peptide Type
Recombinant Proteins
Gene
SFRP5
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
33.8, 32.7 & 30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-40 kDa, reducing conditions
Publications
Read Publications using
6266-SF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human sFRP-5 Protein, CF

  • frizzled-related protein 1b
  • FRP1B
  • FRP-1b
  • SARP3
  • SARP-3
  • secreted apoptosis related protein 3
  • secreted apoptosis-related protein 3
  • secreted frizzled-related protein 5
  • sFRP5
  • sFRP-5

Background

Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP‑2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down‑regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial‑mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down‑regulated in mouse models of obesity and type 2 diabetes (10).
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Leaf, I. et al. (2006) Genesis 44:573.
  6. Satoh, W. et al. (2008) Genesis 46:92.
  7. Li, Y. et al. (2008) Genes Dev. 22:3050.
  8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
  9. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
  10. Ouchi, N. et al. (2010) Science 329:454.
  11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
  12. Zhao, C. et al. (2009) BMC Cancer 9:224.
  13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
  14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

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Publications for sFRP-5 (6266-SF)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, Cell Culture, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
Cell Culture
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 5 of 5.
Publications using 6266-SF Applications Species
A Sahabian, M Sgodda, O Naujok, R Dettmer, J Dahlmann, F Manstein, T Cantz, R Zweigerdt, U Martin, R Olmer Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential Cells, 2019;8(12):. 2019 [PMID: 31817235] (Bioassay, Human) Bioassay Human
DT Au, M Migliorini, DK Strickland, SC Muratoglu Macrophage LRP1 Promotes Diet-Induced Hepatic Inflammation and Metabolic Dysfunction by Modulating Wnt Signaling Mediators Inflamm., 2018;2018(0):7902841. 2018 [PMID: 30524198] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
YK Cho, YM Kang, SE Lee, Y Lee, SM Seol, WJ Lee, JY Park, CH Jung Effect of SFRP5 (Secreted Frizzled-Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With Arterial Stiffness in Human Subjects Arterioscler. Thromb. Vasc. Biol., 2018;0(0):. 2018 [PMID: 29674475] (Cell Culture, Human) Cell Culture Human
Carstensen M, Wiza C, Rohrig K, Fahlbusch P, Roden M, Herder C, Ouwens D Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells. PLoS ONE, 2014;9(1):e85906. 2014 [PMID: 24465779] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol SFRP5
Uniprot