CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB134-500 | |||
| Carrier Free | MAB134-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF134-SP | |||
1341-WF) was serially diluted and captured by Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB1342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human WIF‑1 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human WIF‑1 (Catalog # 1341-WF) was serially diluted and captured by Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB1342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human WIF‑1 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1342-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF135-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB135-500 | |||
| Carrier Free | MAB135-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22234-50ul | |||
1341-WF) was serially diluted and captured by Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB1342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human WIF‑1 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human WIF‑1 (Catalog # 1341-WF) was serially diluted and captured by Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB1342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human WIF‑1 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
![Western Blot: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1G5-Western-Blot-NBP1-51658-img0006.jpg "Western Blot: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1G5-Immunocytochemistry-Immunofluorescence-NBP1-51658-img0007.jpg)
![Immunohistochemistry-Paraffin: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis of ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1G5-Immunohistochemistry-Paraffin-NBP1-51658-img0005.jpg)
![Western Blot: WIF-1 Antibody (1X6C9) [NBP3-16580] - Western blot analysis of extracts of various cell lines, using WIF-1 Rabbit mAb (NBP3-16580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1X6C9-Western-Blot-NBP3-16580-img0001.jpg "Western Blot: WIF-1 Antibody (1X6C9) [NBP3-16580] - Western blot analysis of extracts of various cell lines, using WIF-1 Rabbit mAb (NBP3-16580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western blot analysis on different lysates with NBP3-33102 at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: SW480 cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: Mouse heart tissue lysate (40 µg/Lane) Lane 7: Mouse lung tissue lysate (40 µg/Lane) Lane 8: Rat lung tissue lysate (40 µg/Lane) Lane 9: Human lung tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33102 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33102_-western-blot-639204595242248167.jpg "Western blot analysis on different lysates with NBP3-33102 at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: SW480 cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: Mouse heart tissue lysate (40 µg/Lane) Lane 7: Mouse lung tissue lysate (40 µg/Lane) Lane 8: Rat lung tissue lysate (40 µg/Lane) Lane 9: Human lung tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33102 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: WIF-1 Antibody (SR1697) [NBP3-22234] - Western blot analysis using NBP3-22234 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22234_rabbit-wif-1-mab-sr1697-57202317161824.jpg "Western Blot: WIF-1 Antibody (SR1697) [NBP3-22234] - Western blot analysis using NBP3-22234 in HeLa cell lysate.")
![Western Blot: WIF1 Antibody [NBP1-79491] - Human Muscle lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/WIF1-Antibody-Western-Blot-NBP1-79491-img0002.jpg "Western Blot: WIF1 Antibody [NBP1-79491] - Human Muscle lysate, concentration 0.2-1 ug/ml.")
