Wee1 Overexpression Lysate (Native)

Images

 
Western Blot: Wee1 Overexpression Lysate (Adult Normal) [NBP2-08009] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Wee1.

Product Details

Summary
Applications WB

Order Details

Wee1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_003381. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Wee1, mRNA.
Gene
WEE1

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
71.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Wee1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Wee1 Overexpression Lysate (Native)

  • DKFZp686I18166
  • EC 2.7.10.2
  • FLJ16446
  • WEE1 homolog (S. pombe)
  • wee1+ (S. pombe) homolog
  • WEE1+ homolog
  • Wee1A kinase
  • WEE1A
  • WEE1hu
  • wee1-like protein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF888
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-33466
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33687
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85727
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-61243
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-02275
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-37507
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-81021
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB1489
Species: Hu
Applications: WB
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, IHC-FrFl
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56651
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, IHC-P
MAB1626
Species: Hu
Applications: WB, ICC
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP

Publications for Wee1 Lysate (NBP2-08009) (0)

There are no publications for Wee1 Lysate (NBP2-08009).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Wee1 Lysate (NBP2-08009) (0)

There are no reviews for Wee1 Lysate (NBP2-08009). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Wee1 Lysate (NBP2-08009) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Wee1 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for Wee1 Lysate (NBP2-08009)

Discover related pathways, diseases and genes to Wee1 Lysate (NBP2-08009). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Wee1 Lysate (NBP2-08009)

Discover more about diseases related to Wee1 Lysate (NBP2-08009).
 

Pathways for Wee1 Lysate (NBP2-08009)

View related products by pathway.

PTMs for Wee1 Lysate (NBP2-08009)

Learn more about PTMs related to Wee1 Lysate (NBP2-08009).
 

Research Areas for Wee1 Lysate (NBP2-08009)

Find related products by research area.

Blogs on Wee1.

Funny Protein Names Infographic
This is not a joke, these proteins with funny names actually do exist. View our list of six proteins with funny and unusual names including: Bambi, Yippee-like 3, Wee1, SPAM1, SPOCK1 and Bagpipe homeobox protein homolog 1. Learn more about their fu...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol WEE1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-08009 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Wee1 Recombinant Protein
H00007465-P01-10ug
ATM Antibody
NB100-104
ATM Antibody
NB100-104
ATM Antibody
NB100-104
Wee1 Antibody
H00007465-B01P
Wee1 Antibody
H00007465-B01P