WBSCR22 Antibody - BSA Free

Western Blot: WBSCR22 Antibody [NBP1-31454] - Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: WBSCR22 Antibody [NBP1-31454] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: WBSCR22 protein stained by WBSCR22 antibody diluted at 1:200. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [9810] diluted at 1:20000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: WBSCR22 Antibody [NBP1-31454] - CA922 xenograft, using WBSCR22 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: WBSCR22 Antibody [NBP1-31454] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of WBSCR22 protein by 2. 5 ug of WBSCR22 antibody 12% SDS-PAGE gel.

Immunohistochemistry-Paraffin: WBSCR22 Antibody [NBP1-31454] - Mouse kidney. WBSCR22 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WBSCR22 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WBSCR22 Antibody - BSA Free (NBP1-31454) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-WBSCR22 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human WBSCR22. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WBSCR22
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF usage reported in scientific literature. May be useful for IP active ligase. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relat
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for WBSCR22 Antibody - BSA Free

• EC 2.1.1.-
• FLJ44236
• HASJ4442
• HUSSY-3
• MGC19709
• MGC2022
• MGC5140
• PP3381
• WBMT
• Williams Beuren syndrome chromosome region 22 protein
• Williams Beuren syndrome chromosome region 22
• Williams-Beuren candidate region putative methyltransferase
• Williams-Beuren syndrome chromosomal region 22 protein

Background

This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for WBSCR22 Antibody (NBP1-31454)(1)

Publication using NBP1-31454

• Nakazawa Y, Arai H, Fujita N. The novel metastasis promoter Merm1/Wbscr22 enhances tumor cell survival in the vasculature by suppressing Zac1/p53-dependent apoptosis. Cancer Res 2011-02-01 [PMID: 21148752] (IF/IHC, ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: WBSCR22