XAB1 Antibody (3E1) - Azide and BSA Free

Images

 
Western Blot: XAB1 Antibody (3E1) [H00011321-M01] - XAB1 monoclonal antibody (M01), clone 3E1 Analysis of XAB1 expression in HL-60.
Immunocytochemistry/ Immunofluorescence: XAB1 Antibody (3E1) [H00011321-M01] - Analysis of monoclonal antibody to XAB1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: XAB1 Antibody (3E1) [H00011321-M01] - Analysis of monoclonal antibody to XAB1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: XAB1 Antibody (3E1) [H00011321-M01] - Analysis of XAB1 expression in transfected 293T cell line by XAB1 monoclonal antibody (M01), clone 3E1.Lane 1: XAB1 transfected lysate(41.7 KDa).Lane 2: ...read more
Sandwich ELISA: XAB1 Antibody (3E1) [H00011321-M01] - Detection limit for recombinant GST tagged XAB1 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

XAB1 Antibody (3E1) - Azide and BSA Free Summary

Immunogen
XAB1 (AAH07451, 9 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ELQASGGPRHPVCLLVLGMAGSGKTTFVQRLTGHLHAQGTPPYVINLDPAVHEVPFPANIDIRDTVKYKEVMKQYGLGPNGGIVTSLNLFATRFDQVMKFIEKAQNMSKY
Specificity
XAB1 - XPA binding protein 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GPN1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for XAB1 Antibody (3E1) - Azide and BSA Free

  • ATP(GTP)-binding protein
  • ATPBD1A
  • GPN-loop GTPase 1
  • GTPase
  • MBD2-interacting protein
  • MBDin
  • MBDINXPA binding protein 1
  • XAB1FLJ51176
  • XPA-binding protein 1

Background

The DNA methylation system is composed of methyl-CpG-binding proteins, as well as of DNA cytosine methyl transferases. Five methyl-CpG binding proteins were isolated: MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene. MBD2a is a component of MeCP1, a large corepressor complex that represses transcription from densely methylated genes. Components of MeCP1 include MBD2, Mi-2, MTA2, MBD3 and HDAC1/2. MIZF and MBDin were isolated as proteins interacting with MBD2. MBDin, in turn, is identical to XPA binding protein 1 (XAB1). At the N erminus, the protein contains the consensus sequence for a GTP binding site. At the C terminus, the protein is characterized by an acidic domain containing a cluster of acidic amino acid residues. The transcriptional repression by MBD2 at methylated promoters is relieved by MBdin. XAB1 is expressed ubiquitously, and may be involved in nuclear localization of XPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional XAB1 Products

Array H00011321-M01

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Bioinformatics

Gene Symbol GPN1
Entrez
Uniprot