XAB1 Antibody (3E1)

Western Blot: XAB1 Antibody (3E1) [H00011321-M01] - XAB1 monoclonal antibody (M01), clone 3E1 Analysis of XAB1 expression in HL-60.

Immunocytochemistry/Immunofluorescence: XAB1 Antibody (3E1) [H00011321-M01] - Analysis of monoclonal antibody to XAB1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: XAB1 Antibody (3E1) [H00011321-M01] - Analysis of monoclonal antibody to XAB1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.

Western Blot: XAB1 Antibody (3E1) [H00011321-M01] - Analysis of XAB1 expression in transfected 293T cell line by XAB1 monoclonal antibody (M01), clone 3E1.Lane 1: XAB1 transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: XAB1 Antibody (3E1) [H00011321-M01] - Detection limit for recombinant GST tagged XAB1 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

XAB1 Antibody (3E1) Summary

• Immunogen: XAB1 (AAH07451, 9 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ELQASGGPRHPVCLLVLGMAGSGKTTFVQRLTGHLHAQGTPPYVINLDPAVHEVPFPANIDIRDTVKYKEVMKQYGLGPNGGIVTSLNLFATRFDQVMKFIEKAQNMSKY
• Specificity: XAB1 - XPA binding protein 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for XAB1 Antibody (3E1)

• ATP(GTP)-binding protein
• ATPBD1A
• GPN-loop GTPase 1
• GTPase
• MBD2-interacting protein
• MBDin
• MBDINXPA binding protein 1
• XAB1FLJ51176
• XPA-binding protein 1

Background

The DNA methylation system is composed of methyl-CpG-binding proteins, as well as of DNA cytosine methyl transferases. Five methyl-CpG binding proteins were isolated: MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene. MBD2a is a component of MeCP1, a large corepressor complex that represses transcription from densely methylated genes. Components of MeCP1 include MBD2, Mi-2, MTA2, MBD3 and HDAC1/2. MIZF and MBDin were isolated as proteins interacting with MBD2. MBDin, in turn, is identical to XPA binding protein 1 (XAB1). At the N erminus, the protein contains the consensus sequence for a GTP binding site. At the C terminus, the protein is characterized by an acidic domain containing a cluster of acidic amino acid residues. The transcriptional repression by MBD2 at methylated promoters is relieved by MBdin. XAB1 is expressed ubiquitously, and may be involved in nuclear localization of XPA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPN1
• Entrez:
  • 11321(Human)
• Uniprot:
  • AAH07451(Human)