WARP/VWA1 Antibody (559915)

Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human WARP Monoclonal Antibody (Catalog # MAB6189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for WARP at approximately 50, 40 and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 559915
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

WARP/VWA1 Antibody (559915) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human WARP
  Arg19-Pro445
  Accession # Q6PCB0
• Specificity: Detects human WARP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) WARP, rmCollagen II, or recombinant bovine Collagen I is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VWA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WARP/VWA1 Antibody (559915)

• DKFZp761O051
• von Willebrand factor A domain containing 1
• von Willebrand factor A domain-containing protein 1
• von Willebrand factor A domain-related protein
• VWA1
• WARP

Background

Von Willebrand factor A (vWFA) domain-related protein (WARP) is a 50 kDa glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted human WARP contains a vWFA domain (aa 34‑213), two fibronectin type III domains (aa 211‑301 and 331‑421) that likely bind to the GAG modification of perlecan, and one potential site for N-linked glycosylation. There is one alternate start site at Met213. Mature human WARP shares 72% aa sequence identity with mature mouse and rat WARP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VWA1
• Uniprot:
  • Q6PCB0()