WAPL Antibody (2B3) - Azide and BSA Free

Western Blot: WAPL Antibody (2B3) [H00023063-M02] - Analysis of KIAA0261 expression in transfected 293T cell line by KIAA0261 monoclonal antibody (M02), clone 2B3.Lane 1: KIAA0261 transfected lysate(45.6 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: WAPL Antibody (2B3) [H00023063-M02] - Analysis of WAPAL transfected lysate using anti-WAPAL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WAPAL monoclonal antibody.

Sandwich ELISA: WAPL Antibody (2B3) [H00023063-M02] - Detection limit for recombinant GST tagged KIAA0261 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 2B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

WAPL Antibody (2B3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse WAPL Antibody (2B3) - Azide and BSA Free (H00023063-M02) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KIAA0261 (AAH17393, 1 a.a. ~ 402 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC
• Specificity: KIAA0261 (2B3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: WAPL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WAPL Antibody (2B3) - Azide and BSA Free

• FOEKIAA0261friend of EBNA2 (Epstein-Barr virus nuclear protein 2)
• Friend of EBNA2 protein
• WAPLwings apart-like protein homolog
• wings apart-like homolog (Drosophila)

Background

The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weak expression in all other normal and tumor tissues examined. hWAPL expression in benign epithelia was confined to the basal cell layers, whereas in dysplasias it increasingly appeared in more superficial cell layers and showed a significant correlation with severity of dysplasia. Diffuse hWAPL expression was found in all invasive squamous cell carcinomas examined. These results demonstrate that hWAPL is associated with cell growth, and the hWAPL expression may play a significant role in cervical carcinogenesis and tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WAPL
• Entrez:
  • 23063(Human)
• Uniprot:
  • AAH17393(Human)