VSIG3/IGSF11 Antibody - BSA Free

Analysis in human cell lines SK-MEL-30 and U-251MG using Anti-IGSF11 antibody. Corresponding IGSF11 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Staining of human cell line A549 shows localization to nucleus, cytosol & cell junctions.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VSIG3/IGSF11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit VSIG3/IGSF11 Antibody - BSA Free (NBP1-92021) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PPKCSSAKAFHTEISSSDNNTLTSSNAYNSRYWSNNPKVHRNTESVSHFSDLGQSFSFHSGNANIPSIYANGTHLVPGQHKTLVVTANRGSSPQVMSRSNGSVSRKP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGSF11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  VSIG3/IGSF11 Protein (NBP1-92021PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for VSIG3/IGSF11 Antibody - BSA Free

• Brain and testis-specific immunoglobulin superfamily protein
• BTIGSF
• BT-IgSF
• cancer/testis antigen 119
• CT119
• CXADR like 1
• CXADRL1
• IGSF11
• Igsf13
• immunoglobulin superfamily member 11
• immunoglobulin superfamily, member 11
• MGC35227
• V-set and immunoglobulin domain containing 3
• V-set and immunoglobulin domain-containing protein 3
• VSIG3
• VSIG3brain and testis-specific immunoglobin superfamily protein

Background

IGSF11 is an immunoglobulin (Ig) superfamily member that is preferentially expressed in brain and testis. It sharessignificant homology with coxsackievirus and adenovirus receptor (CXADR; MIM 602621) and endothelial cell-selectiveadhesion molecule (ESAM).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGSF11