VRK1 Antibody - BSA Free

Western Blot: VRK1 Antibody [NBP2-93500] - Analysis of extracts of various cell lines, using VRK1 . Exposure time: 90s.

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP2-93500] - Paraffin-embedded rat lung using VRK1 .

Immunohistochemistry-Paraffin: VRK1 Antibody [NBP2-93500] - Paraffin-embedded mouse lung using VRK1 .

Western Blot: VRK1 Antibody - BSA Free [NBP2-93500] - Western blot analysis of lysates from wild type(WT) and VRK1 knockout (KO) 293T cells, using [KO Validated] VRK1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VRK1 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1). GHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLKAKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRKRVQK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VRK1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:500
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for VRK1 Antibody - BSA Free

• EC 2.7.11.1
• MGC117401
• MGC138280
• MGC142070
• PCH1
• serine/threonine-protein kinase VRK1
• vaccinia related kinase 1
• vaccinia virus B1R-related kinase 1
• Vaccinia-related kinase 1
• VRK1

Background

VRK1 encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VRK1