Von Hippel Lindau Antibody

Product Discontinued

Von Hippel Lindau Antibody Summary

• Immunogen: A synthetic peptide common to both human and mouse Von Hippel Lindau protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VHL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:1000
• Application Notes: This Von Hippel Lindau antibody is useful for Immunoprecipitation (PMID 21658608) and Western blot. In Western blot a band is observed ~21 kDa. The theoretical molecular weight of VHL is 24 kDa. However, different migrating species ranging from 21-30 kDa have been observed using antibodies to VHL, and may result from a variety of factors including alternatively spliced VHL mRNAs and protein degradation. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 28643803). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID:31928714).
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Von Hippel Lindau Antibody

• elongin binding protein
• HRCA1
• Protein G7
• pVHL
• RCA1
• VHL
• VHL1
• von Hippel-Lindau disease tumor suppressor
• von Hippel-Lindau syndrome
• von Hippel-Lindau tumor suppressor

Background

The von Hippel-Lindau (VHL) protein is a tumor suppressor that is highly conserved. VHL binds to transcription factor Elongin B and C subunits, and appears to prevent Elongin A from associating with the B and C subunits, thereby inhibiting the transcriptional activity of Elongin. The ability of VHL to block Elongin function may play a role in normal cell growth regulation. Mutated VHL sequences lose their ability to bind to Elongin subunits B and C, suggesting that the tumor suppression function of VHL may be linked to its ability to bind to Elongin. Likewise, the introduction of wild-type VHL suppresses growth in tumor cell lines lacking normal expression of VHL genes. The product of the VHL gene has multiple reported functions, the best characterized of which is its role as the recognition component of an ubiquitin E3 ligase complex responsible for mediating oxygen-dependent destruction of hypoxia-inducible factor-alpha (HIF-alpha) subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Von Hippel Lindau Antibody (NB100-485)(9)

Publications using NB100-485

• Aggarwal R, Zou Y, Luchtel R et al. Functional succinate dehydrogenase deficiency is a pathognomonic adverse feature of clear cell renal cancer Proc Natl Acad Sci U S A 2021-09-23 [PMID: 34551979]
• Kaiho-Soma A, Akizuki Y, Igarashi K et al. TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains Molecular cell 2021-02-03 [PMID: 33567268]
• Fu RJ, He W, Wang XB et al. DNMT1-maintained hypermethylation of Kruppel-like factor 5 involves in the progression of clear cell renal cell carcinoma Cell Death Dis 2017-07-27 [PMID: 28749461] (WB, Human)
  
  Details:
  This Novus VHL antibody was used to measure VHL expression in different ccRCC lines and helped to show that VHL expression down regulates KLF5 expression in ccRCC cell lines that are VHL deficient.
• Alahari S, Post M, Caniggia I. Jumonji Domain Containing Protein 6 - A novel oxygen sensor in the human placenta. Endocrinology 2015-06-02 [PMID: 26037477] (Human)
• Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1 Mol Cell 2011-06-10 [PMID: 21658608] (IP, WB, Human)
• Alahari S. The Dynamic Role of Jumonji C Domain Containing Protein 6 in Placental Development and Disease Thesis 2017-01-01 (Human)
• Wang F, Wang LS, Gao YH, Yao XD VHL enhances 9-cis-retinoic acid treatment by down-regulating retinoid X receptor alpha in renal cell carcinomas Biochem. Biophys. Res. Commun. 2020-01-09 [PMID: 31928714] (WB, IHC-P, Human)
• Gao YH, Wu ZX, Xie LQ et al. VHL deficiency augments anthracycline sensitivity of clear cell renal cell carcinomas by down-regulating ALDH2 Nat Commun. 2017-06-14 [PMID: 28643803] (WB, Chemotaxis, Human)
• Esteban, MA et al. Regulation of E-cadherin expression by VHL hypoxia-inducible factor. Cancer Res 66(7): 3567-3575. 2006-01-01 [PMID: 16585181] (WB, Human)

Reviews for Von Hippel Lindau Antibody (NB100-485) (5)

Average Rating: 4.8

(Based on 5 reviews)

We have 5 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/03/2020

Summary

• Application: Western Blot
• Sample Tested: hela cell,Human breast cancer cell lysate (MCF-7)
• Species: Human
• Lot: 091504

reviewed by: Verified Customer WB Human 11/06/2018

Summary

• Application: Western Blot
• Sample Tested: A549 cells,hela cell
• Species: Human
• Lot: 091504

reviewed by: Verified Customer WB Human 10/22/2018

Summary

• Application: Western Blot
• Sample Tested: A549 cells,MDA MB 231 cells
• Species: Human
• Lot: 091504

reviewed by: Verified Customer WB Human 04/30/2018

Summary

• Application: Western Blot
• Sample Tested: 20 ug whole cell lysate
• Species: Human
• Lot: A-2

reviewed by: Verified Customer WB Human 08/10/2010

Summary

• Application: Western Blot
• Sample Tested: 293T Cell Lysate, Sample Amount: 50 ug
• Species: Human

FAQs for Von Hippel Lindau Antibody (NB100-485). (Showing 1 - 1 of 1 FAQs).

1. I'm not sure that this Ab can distinghish between VHL wild-type and the VHL mutated form that is present in renal cell carcinoma patients. Do you have some evidence that it can?
   • We have not actually tested if this antibody can distinguish between the two forms.

Bioinformatics

• Gene Symbol: VHL
• Entrez:
  • 7428(Human)
• Uniprot:
  • P40337(Human)