Vitronectin Antibody

Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vitronectin stained by Vitronectin antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Human colon carcinoma, using Vitronectin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Vitronectin Antibody [NBP2-20866] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (NBP2-20866) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Vitronectin antibody detects Vitronectin protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vitronectin antibody (NBP2-20866) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Vitronectin Antibody Summary

• Description: Novus Biologicals Rabbit Vitronectin Antibody (NBP2-20866) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Vitronectin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary.
• Localization: Secreted; extracellular space
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VTN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Vitronectin Antibody

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Vitronectin Antibody (NBP2-20866)(2)

Publications using NBP2-20866

• Stuart CH, Riley KR, Boyacioglu O et al. Selection of a Novel Aptamer Against Vitronectin Using Capillary Electrophoresis and Next Generation Sequencing. Mol Ther Nucleic Acids. 2016-11-15 [PMID: 27845768] (IF/IHC, Human)
• Nystrom A, Thriene K, Mittapalli V et al. Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms EMBO Mol Med. 2015-07-20 [PMID: 26194911] (WB, Human)

Bioinformatics

• Gene Symbol: VTN
• Uniprot:
  • P04004(Human)