Vitronectin Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Vitronectin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Vitronectin was detected in immersion fixed paraffin-embedded sections of human bladder using Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular matrix. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows human serum, loaded at a 1:300 dilution. A specific band was detected for Vitronectin at approximately 76 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Vitronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2349) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Vitronectin Antibody [Unconjugated] Summary

• Immunogen: Human plasma-derived human Vitronectin
• Specificity: Detects human Vitronectin in direct ELISAs and Western blot.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VTN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vitronectin Antibody [Unconjugated]

• Complement S-protein
• epibolin
• Serum Spreading Factor
• Serum-spreading factor
• Somatomedin B
• S-protein
• V75
• vitronectin (serum spreading factor, somatomedin B, complement S-protein)
• Vitronectin
• VN
• VNT
• VTN

Background

VN/Vitronectin (vitro-nectin: Latin for "glass" and "to bind to", respectively) is a 65-78 kDa circulating glycoprotein that was initially named for to its ability to 1) bind to glass beads, and 2) attach to cell membranes. Also known as somatomedin-B and Serum Spreading Factor, vitronectin is a matricellular protein that belongs to a group of RGD-type adhesive molecules. It is secreted by hepatocytes and circulates as a monomer/homodimer. Upon binding to ECM or PIA-1, it oligomerizes and becomes active. VN acts as a promoter of cell migration and ECM remodeling. It does so by binding to HSPG in the ECM which exposes binding sites for integrins and uPAR on cells. In this context, VN allows for the formation of a uPAR:soluble uPAR:uPA complex that results in the proteolysis of pericellular matrix and subsequent cell migration. Vitronectin also serves as a receptor for circulating PAI-1. When bound, VN forms oligomers that facilitate subsequent binding to vimentin, a cellular intermediate filament. The vimentin:VN:PAI-1 complex is active, and blocks the enzymatic degradation of vascular clots. Human secreted vitronectin is a 459 amino acid (aa) glycoprotein. It can exist as either a 77-78 kDa monomer, or an internal disulfide-linked 65 kDa:10 kDa heterodimer that arises from proteolytic cleavage between Ala398Thr399. VN has multiple domains, including a somatomedin B domain that binds uPAR and PAI-1 (aa 1-40), an RGD motif (aa 64-66) and a heparin-binding region (aa 340-380). VN is sulfated, N- and O-glycosylated, and phosphorylated on at least five Thr/Ser based sites. Over aa 20-29 (i.e.‑DQESCKGRCT), human and mouse VN are identical in their amino acid sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vitronectin Antibody (AF2349)(1)

Publication using AF2349

• Rust, R;Sagare, AP;Kisler, K;Kim, Y;Zhang, M;Griffin, C;Wang, Y;Clementel, V;Torres-Sepulveda, C;Tcw, J;Zlokovic, BV;Coba, MP; Molecular signature and functional properties of human pluripotent stem cell-derived brain pericytes bioRxiv : the preprint server for biology 2025-04-28 [PMID: 40291694] (Proximity Ligation Assay (PLA), Mouse)

Bioinformatics

• Gene Symbol: VTN