VEGFR2/KDR/Flk-1 Antibody (EIC)

Images

 
Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Detection of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing ...read more
Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [Allophycocyanin] [NB200-208APC] - HUVECs were unstained (left) or stained (right) with VEGFR-2/KDR. Image ...read more
Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.
Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Staining of VEGFR-2/KDR expression in HUVE cells.
ELISA: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Functional ELISA with anti-human VEGFR-2/KDR. Recombinant human soluble KDR (D7) was coated with increasing amounts in a 96 well microtiter plate. The antibody was ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
EIC
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

VEGFR2/KDR/Flk-1 Antibody (EIC) Summary

Description
PLEASE NOTE: 0.02mg size is provided in liquid format. 0.1mg size will ship lyophilized. Please see Reconstitution Instructions.
Immunogen
recombinant human activin-C
Localization
Type I membrane protein.
Marker
Endothelial Cell Marker
Specificity
The monoclonal will detect native human VEGFR-2 / KDR in ELISA experiments and on the surface of different human cell types.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1 - 15 ug/ml
  • Flow Cytometry 3 - 15 ug/ml
  • Immunocytochemistry/Immunofluorescence 6 - 30 ug/ml
  • Immunohistochemistry 6 - 30 ug/ml
  • Immunohistochemistry-Paraffin 6 - 30 ug/ml
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Control
VEGFR2/KDR/Flk-1 Overexpression Lysate
Publications
Read Publications using
NB200-208 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 20837901).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute the antibody with 500 ul sterile PBS and the final concentration is 200 ug/ml.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (EIC)

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready

Publications for VEGFR2/KDR/Flk-1 Antibody (NB200-208)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Flow, ICC/IF.


Filter By Application
Flow
(1)
ICC/IF
(1)
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Filter By Species
Rat
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGFR2/KDR/Flk-1 Antibody (NB200-208) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-208AF350
Alexa Fluor 405 NB200-208AF405
Alexa Fluor 488 NB200-208AF488
Alexa Fluor 532 NB200-208AF532
Alexa Fluor 594 NB200-208AF594
Alexa Fluor 647 NB200-208AF647
Alexa Fluor 700 NB200-208AF700
Alexa Fluor 750 NB200-208AF750
Allophycocyanin NB200-208APC
Allophycocyanin/Cy7 NB200-208APCCY7
Biotin NB200-208B
DyLight 350 NB200-208UV
DyLight 405 NB200-208V
DyLight 488 NB200-208G
DyLight 550 NB200-208R
DyLight 594 NB200-208DL594
DyLight 650 NB200-208C
DyLight 680 NB200-208FR
DyLight 755 NB200-208IR
FITC NB200-208F
HRP NB200-208H
Janelia Fluor 549 NB200-208JF549
Janelia Fluor 646 NB200-208JF646
PE NB200-208PE
PE/Atto594 NB200-208PEATT594
PE/Cy5.5 NB200-208PECY55
PE/Cy7 NB200-208PECY7
PerCP NB200-208PCP

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Bioinformatics Tool for VEGFR2/KDR/Flk-1 Antibody (NB200-208)

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Bioinformatics

Gene Symbol KDR
Entrez
Uniprot