VEGFR2/KDR/Flk-1 Antibody (EIC) - Azide and BSA Free

Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.

Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Staining of VEGFR-2/KDR expression in HUVE cells.

ELISA: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Functional ELISA with anti-human VEGFR-2/KDR. Recombinant human soluble KDR (D7) was coated with increasing amounts in a 96 well microtiter plate. The antibody was used at 1ug/ml.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: EIC
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

VEGFR2/KDR/Flk-1 Antibody (EIC) - Azide and BSA Free Summary

• Description: PLEASE NOTE: 0.02mg size is provided in liquid format. 0.1mg size will ship lyophilized. Please see Reconstitution Instructions.
• Immunogen: Recombinant human soluble extracellular KDR (D7)
• Localization: Type I membrane protein.
• Marker: Endothelial Cell Marker
• Specificity: The monoclonal will detect native human VEGFR-2 / KDR in ELISA experiments and on the surface of different human cell types.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1 - 10 ug/ml
  • Flow Cytometry 2- 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 6 - 30 ug/ml
  • Immunohistochemistry 6 - 30 ug/ml
  • Immunohistochemistry-Paraffin 6 - 30 ug/ml
  • Western Blot 2 - 5 ug/ml
• Application Notes: This antibody is Cytof ready.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 20837901).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (EIC) - Azide and BSA Free

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR2/KDR/Flk-1 Antibody (NB200-208)(3)

Publications using NB200-208

• Parlak H, Ozkan A, Sinen O et al. Adropin increases with swimming exercise and exerts a protective effect on the brain of aged rats Experimental gerontology 2022-11-01 [PMID: 36216130] (IHC-P)
• Frederick JR, Fitzpatrick JR 3rd, McCormick RC et al. Stromal Cell-Derived Factor-1alpha Activation of Tissue-Engineered Endothelial Progenitor Cell Matrix Enhances Ventricular Function After Myocardial Infarction by Inducing Neovasculogenesis. Circulation. 2010-09-14 [PMID: 20837901] (ICC/IF, Rat)
• Atluri Pavan, Panlilio Corinna M, Liao George P et al. Acute myocardial rescue with endogenous endothelial progenitor cell therapy. Heart, Lung & Circulation 2010-01-01 [PMID: 20719564] (FLOW, Rat)

Bioinformatics

• Gene Symbol: KDR
• Entrez:
  • 3791(Human)
  • 25589(Rat)
• Uniprot:
  • P35968()