VEGFR2/KDR/Flk-1 Antibody (522302) [Phycoerythrin]

• Applications: Flow
• Clone: 522302
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

VEGFR2/KDR/Flk-1 Antibody (522302) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2/KDR/Flk‑1
  Ala20-Glu762
  Accession # P35918
• Specificity: Detects mouse VEGF R2/KDR/Flk‑1 in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: KDR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

VEGF R2, also known as KDR, Flk-1, and CD309, is a 210-220 kDa type I transmembrane glycoprotein member of the 7-Ig/Class V subfamily that belongs to the tyrosine protein kinase family of molecules (1, 2). It is principally expressed on endothelial cells (EC), but also appears on neurons, mast cells, immature dendritic cells and monocytes (3-6). VEGF R2 serves as the major proangiogenic switch that regulates blood vessel development and repair (1, 2). It is known to bind VEGF-A, -C, and -D. On the cell surface, VEGF R2 either homodimerizes or heterodimerizes with VEGF R3 (1). As a homodimer, it appears to interact with Neuropilin-1 (7). It also interacts with Integrin beta 3 subunit, either through its extracellular domain or cytoplasmic tail (8). This interaction may be necessary for EC activation. Notably, VEGF R2 can also be activated in the absence of a VEGF ligand when under torsion stress and in a complex with CD31 and VE-Cadherin (9). Over amino acids 20-762, mouse VEGF R2 shares 93% and 80% amino acid sequence identity with rat and human VEGF R2, respectively.

1. Smith, G.A. et al. (2015) Biosci. Rep. Aug 18 [ePub ahead of print].
   
2. Shibuya, M. (2013) J. Biochem. 153:13.
3. Le, X-F. et al. (2008) Cell Cycle 7:3747.
4. Okabe, K. et al. (2014) Cell 159:584.
5. Detoraki, A. et al. (2009) J. Allergy Clin. Immunol. 123:1142.
6. Dong, Z. et al. (2009) Curr. Pharm. Des. 15:365.
7. Plein, A. et al. (2014) Microcirculation 21:315.
8. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
9. DuToit, A. (2015) Nat. Rev. Mol. Cell Biol. 16:268.
   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDR