VEGFR2/KDR/Flk-1 Antibody (522302) [Allophycocyanin]

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R2/KDR/Flk-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4432A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 522302
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

VEGFR2/KDR/Flk-1 Antibody (522302) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2/KDR/Flk‑1
  Ala20-Glu762
  Accession # P35918
• Specificity: Detects mouse VEGF R2/KDR/Flk‑1 in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: KDR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (522302) [Allophycocyanin]

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGF R2, also known as KDR, Flk-1, and CD309, is a 210-220 kDa type I transmembrane glycoprotein member of the 7-Ig/Class V subfamily that belongs to the tyrosine protein kinase family of molecules (1, 2). It is principally expressed on endothelial cells (EC), but also appears on neurons, mast cells, immature dendritic cells and monocytes (3-6). VEGF R2 serves as the major proangiogenic switch that regulates blood vessel development and repair (1, 2). It is known to bind VEGF-A, -C, and -D. On the cell surface, VEGF R2 either homodimerizes or heterodimerizes with VEGF R3 (1). As a homodimer, it appears to interact with Neuropilin-1 (7). It also interacts with Integrin beta 3 subunit, either through its extracellular domain or cytoplasmic tail (8). This interaction may be necessary for EC activation. Notably, VEGF R2 can also be activated in the absence of a VEGF ligand when under torsion stress and in a complex with CD31 and VE-Cadherin (9). Over amino acids 20-762, mouse VEGF R2 shares 93% and 80% amino acid sequence identity with rat and human VEGF R2, respectively.

1. Smith, G.A. et al. (2015) Biosci. Rep. Aug 18 [ePub ahead of print].
   
2. Shibuya, M. (2013) J. Biochem. 153:13.
3. Le, X-F. et al. (2008) Cell Cycle 7:3747.
4. Okabe, K. et al. (2014) Cell 159:584.
5. Detoraki, A. et al. (2009) J. Allergy Clin. Immunol. 123:1142.
6. Dong, Z. et al. (2009) Curr. Pharm. Des. 15:365.
7. Plein, A. et al. (2014) Microcirculation 21:315.
8. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
9. DuToit, A. (2015) Nat. Rev. Mol. Cell Biol. 16:268.
   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR2/KDR/Flk-1 Antibody (FAB4432A)(1)

Publication using FAB4432A

• A Filippi, A Constantin, N Alexandru, G Voicu, CA Constantin, D Rebleanu, M Fenyo, D Simionescu, A Simionescu, I Manduteanu, A Georgescu Integrins alpha4beta1 and alphaVbeta3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease Cell Transplant, 2020-01-01;29(0):9636897209462. 2020-01-01 [PMID: 32841051] (Flow Cytometry, Mouse, Transgenic Mouse)

Bioinformatics

• Gene Symbol: KDR
• Uniprot:
  • P35918()