VEGFR1/Flt-1 [p Tyr1213] Antibody [Unconjugated]

Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-VEGFR1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

VEGFR1/Flt-1 [p Tyr1213] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human VEGFR1/Flt-1 Y1213 site
• Modification: p Tyr1213
• Specificity: Detects human VEGFR1/Flt-1 when phosphorylated at Y1213.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 [p Tyr1213] Antibody [Unconjugated]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGFR1, also known as Flt-1, is a receptor tyrosine kinase that contains seven Ig-like repeats in its extracellular domain, a single transmembrane region, and a kinase domain in the intracellular region. Ligand binding induces autophosphorylation on multiple intracellular tyrosine residues. Y1213 is a major binding site for PI3K, Nck, and SHP-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (AF4170)(14)

Publications using AF4170

• 2020-12-17;0(0):. 2020-12-17 [PMID: 33348564] (Western Blot, Human)
• X Wang, X Che, Y Yu, Y Cheng, M Bai, Z Yang, Q Guo, X Xie, D Li, M Guo, K Hou, W Guo, X Qu, L Cao Hypoxia-autophagy axis induces VEGFA by peritoneal mesothelial cells to promote gastric cancer peritoneal metastasis through an integrin &alpha5-fibronectin pathway J Exp Clin Cancer Res, 2020-10-20;39(1):221. 2020-10-20 [PMID: 33081836] (Cell Culture, Human)
• Hailu Wu, Ming Wei, Xinglu Jiang, Jiacheng Tan, Wei Xu, Xiaobo Fan et al. lncRNA PVT1 Promotes Tumorigenesis of Colorectal Cancer by Stabilizing miR-16-5p and Interacting with the VEGFA/VEGFR1/AKT Axis Molecular Therapy - Nucleic Acids 2020-06-05 [PMID: 32276209] (Western Blot, Immunocytochemistry, Human)
• Thomas G. Blanchard, Steven J. Czinn, Vivekjyoti Banerjee, Neha Sharda, Andrea C. Bafford, Fahad Mubariz et al. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer Cancers (Basel) 2019-02-08 [PMID: 30744076]
• Y Zhang, C Cao, J Xin, P Lv, D Chen, S Li, H Yang, C Chen, B Liu, Q Li Treatment with placental growth factor attenuates myocardial ischemia/reperfusion injury PLoS ONE, 2018-09-13;13(9):e0202772. 2018-09-13 [PMID: 30212490] (Western Blot, Mouse)
• TG Blanchard, R Lapidus, V Banerjee, AC Bafford, SJ Czinn, H Ahmed, A Banerjee Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation Cell. Physiol. Biochem., 2018-07-25;48(3):1259-1273. 2018-07-25 [PMID: 30045022] (Western Blot, Human)
• MG Atzori, L Tentori, F Ruffini, C Ceci, L Lisi, E Bonanno, M Scimeca, E Eskilsson, T Daubon, H Miletic, L Ricci Viti, R Pallini, P Navarra, R Bjerkvig, S D'Atri, PM Lacal, G Graziani The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells J. Exp. Clin. Cancer Res., 2017-08-10;36(1):106. 2017-08-10 [PMID: 28797294] (Western Blot, Human)
• R Bhattachar, F Fan, R Wang, X Ye, L Xia, D Boulbes, LM Ellis Intracrine VEGF signalling mediates colorectal cancer cell migration and invasion Br. J. Cancer, 2017-07-25;117(6):848-855. 2017-07-25 [PMID: 28742793] (Western Blot, Human)
• Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth. Clin Cancer Res, 2015-01-23;21(7):1652-64. 2015-01-23 [PMID: 25617424] (Western Blot, Human)
• Huang H, He J, Johnson D, Wei Y, Liu Y, Wang S, Lutty G, Duh E, Semba R Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1alpha-VEGF pathway inhibition. Diabetes, 2014-09-03;64(1):200-12. 2014-09-03 [PMID: 25187372] (IHC, Mouse)
• Meda C, Molla F, De Pizzol M Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages. J. Immunol., 2012-03-21;188(8):4081-92. 2012-03-21 [PMID: 22442441] (Western Blot, Human)
• Attar-Schneider O, Drucker L, Zismanov V Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells. Lab. Invest., 2011-11-14;92(2):178-90. 2011-11-14 [PMID: 22083671] (Western Blot, Human)
• Jemima L. Whyte, Stephen G. Ball, C. Adrian Shuttleworth, Keith Brennan, Cay M. Kielty Density of human bone marrow stromal cells regulates commitment to vascular lineages Stem Cell Research 2011-05-01 [PMID: 21420373] (Western Blot, Immunocytochemistry, Human)
• Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1. J. Biol. Chem., 2008-10-15;283(49):34250-9. 2008-10-15 [PMID: 18922791] (Western Blot, Human)

Bioinformatics

• Gene Symbol: FLT1