VEGFR1/Flt-1 Antibody (49560) [Unconjugated]

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HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt‑1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
49560
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

VEGFR1/Flt-1 Antibody (49560) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB321 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (49560) [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR1/Flt-1 Antibody (MAB321)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Cell Culture, ELISA Development, Flow Cytometry, Neutralization, Western Blot.


Filter By Application
Cell Culture
(1)
ELISA Development
(2)
Flow Cytometry
(4)
Neutralization
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(11)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB321 Applications Species
A Schulz, CC Drost, B Hesse, K Beul, M Brand, GS Di Marco The Soluble Fms-like Tyrosine Kinase-1 Contributes to Structural and Functional Changes in Endothelial Cells in Chronic Kidney Disease International Journal of Molecular Sciences, 2022;23(24):. 2022 [PMID: 36555698] (Cell Culture, Human) Cell Culture Human
Gabriela Odent Grig Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 2016;0(0):. 2016 [PMID: 27943613] (Flow Cytometry, Human) Flow Cytometry Human
Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms. PLoS ONE, 2014;9(4):e93965. 2014 [PMID: 24713821] (Flow Cytometry, Human) Flow Cytometry Human
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011;475(7355):226-30. 2011 [PMID: 21753853] (Neutralization, Human) Neutralization Human
Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. Angiogenesis, 2010;13(4):337-47. 2010 [PMID: 20953695] (ELISA Development, Human) ELISA Development Human
Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells. Cancer Res., 2009;69(5):1976-84. 2009 [PMID: 19223539] (Western Blot, Human) Western Blot Human
Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels. Leuk. Res., 2008;32(8):1244-58. 2008 [PMID: 18304628] (Flow Cytometry, Human) Flow Cytometry Human
Lin YL, Liang YC, Chiang BL Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. J. Leukoc. Biol., 2007;82(6):1473-80. 2007 [PMID: 17761954] (Neutralization, Human) Neutralization Human
Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. Stem Cells, 2007;25(8):1862-71. 2007 [PMID: 17446561] (Flow Cytometry, Human) Flow Cytometry Human
Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis. Blood, 2007;109(4):1479-89. 2007 [PMID: 17038538] (Neutralization, Human) Neutralization Human
Show All 11 Publications.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FLT1
Uniprot