VEGFR1/Flt-1 Antibody (49560) [Unconjugated]

HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt‑1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 49560
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VEGFR1/Flt-1 Antibody (49560) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
  Ser27-His687
  Accession # NP_001153392
• Specificity: Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (49560) [Unconjugated]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (MAB321)(14)

Publications using MAB321

• A Schulz, CC Drost, B Hesse, K Beul, M Brand, GS Di Marco The Soluble Fms-like Tyrosine Kinase-1 Contributes to Structural and Functional Changes in Endothelial Cells in Chronic Kidney Disease International Journal of Molecular Sciences, 2022-12-16;23(24):. 2022-12-16 [PMID: 36555698] (Cell Culture, Human)
• ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020-05-04;11(5):307. 5/4/2020 [PMID: 32366833]
• Gabriela Odent Grig Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 2016-12-12;0(0):. 2016-12-12 [PMID: 27943613] (Flow Cytometry, Human)
• Kirsten R. Palmer, Tu’uhevaha J. Kaitu’u-Lino, Roxanne Hastie, Natalie J. Hannan, Louie Ye, Natalie Binder et al. Placental-Specific sFLT-1 e15a Protein Is Increased in Preeclampsia, Antagonizes Vascular Endothelial Growth Factor Signaling, and Has Antiangiogenic Activity Hypertension 2015-12-01 [PMID: 26416849]
• Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms. PLoS ONE, 2014-04-08;9(4):e93965. 2014-04-08 [PMID: 24713821] (Flow Cytometry, Human)
• Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011-07-13;475(7355):226-30. 2011-07-13 [PMID: 21753853] (Neutralization, Human)
• Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model. Angiogenesis, 2010-10-16;13(4):337-47. 2010-10-16 [PMID: 20953695] (ELISA Development, IHC, Human)
• Ling Xu, Martin Hausmann, Wolfgang Dietmaier, Silvia Kellermeier, Theresa Pesch, Manuela Stieber-Gunckel et al. Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines BMC Cancer 2010-12-01 [PMID: 20565817]
• Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells. Cancer Res., 2009-02-17;69(5):1976-84. 2009-02-17 [PMID: 19223539] (Western Blot, Human)
• Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels. Leuk. Res., 2008-03-04;32(8):1244-58. 2008-03-04 [PMID: 18304628] (Flow Cytometry, Human)
• Lin YL, Liang YC, Chiang BL Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. J. Leukoc. Biol., 2007-08-30;82(6):1473-80. 2007-08-30 [PMID: 17761954] (Neutralization, Human)
• Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. Stem Cells, 2007-04-19;25(8):1862-71. 2007-04-19 [PMID: 17446561] (Flow Cytometry, Human)
• Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis. Blood, 2006-10-12;109(4):1479-89. 2006-10-12 [PMID: 17038538] (Neutralization, Human)
• Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur. J. Clin. Invest., 2003-10-01;33(10):883-90. 2003-10-01 [PMID: 14511360] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • NP_001153392()