VEGFR1/Flt-1 Antibody (49560) [Phycoerythrin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R1/Flt-1 PE-conjugated Monoclonal Antibody (Catalog # FAB321P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 49560
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

VEGFR1/Flt-1 Antibody (49560) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1
  Ser27-His687
  Accession # NP_001153392
• Specificity: Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody (49560) [Phycoerythrin]

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR1/Flt-1 Antibody (FAB321P)(23)

Publications using FAB321P

• Liu, X;Fang, Y;Imoukhuede, PI; Cultured endothelial cells present organ-specific RTK distributions: advancing receptor measurement and data standardization via quantitative flow cytometry bioRxiv : the preprint server for biology 2025-02-28 [PMID: 40060580] (Flow Cytometry, Human)
• Stephane Oudard, Nadine Benhamouda, Bernard Escudier, Patrice Ravel, Thi Tran, Emeline Levionnois et al. Decrease of Pro-Angiogenic Monocytes Predicts Clinical Response to Anti-Angiogenic Treatment in Patients with Metastatic Renal Cell Carcinoma Cells 12/22/2021 [PMID: 35011579]
• MT Bergamo, Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, 1011 N. University Rm. G049, Ann Arbor, MI 48109-1078, USA, Z Zhang, TM Oliveira, JE Nör VEGFR1 primes a unique cohort of dental pulp stem cells for vasculogenic differentiation European Cells and Materials 3/16/2021 [PMID: 33724439]
• M Witkowska, A Majchrzak, B Cebula-Obr, E Wawrzyniak, T Robak, P Smolewski The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia Tumour Biol, 2017-03-01;39(3):1010428317694. 2017-03-01 [PMID: 28349818] (Flow Cytometry, Human)
• P Guo, J Yang, DR Bielenberg, D Dillon, D Zurakowski, MA Moses, DT Auguste A quantitative method for screening and identifying molecular targets for nanomedicine J Control Release, 2017-03-22;0(0):. 2017-03-22 [PMID: 28341549] (Flow Cytometry, Human)
• Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth. Clin Cancer Res, 2015-01-23;21(7):1652-64. 2015-01-23 [PMID: 25617424] (Flow Cytometry, Human)
• Fiorio Pla , Alessand, Brossa , Alessia, Bernardini , Michela, Genova , Tullio, Grolez , Guillaum, Villers , Arnaud, Leroy , Xavier, Prevarskaya , Natalia, Gkika , Dimitra, Bussolati , Benedett Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib. BMC Cancer, 2014-12-12;14(0):939. 2014-12-12 [PMID: 25494980] (Flow Cytometry, Human)
• Norton , Kerri-An, Han , Zheyi, Popel , Aleksand, Pandey , Niranjan Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells. Onco Targets Ther, 2014-09-09;7(0):1571-82. 2014-09-09 [PMID: 25228815] (Flow Cytometry, Human)
• Mesti T, Savarin P, Triba M, Le Moyec L, Ocvirk J, Banissi C, Carpentier A Metabolic impact of anti-angiogenic agents on U87 glioma cells. PLoS ONE, 2014-06-12;9(6):e99198. 2014-06-12 [PMID: 24922514] (Flow Cytometry, Human)
• Stamati K, Priestley J, Mudera V, Cheema U Laminin promotes vascular network formation in 3D in vitro collagen scaffolds by regulating VEGF uptake. Exp Cell Res, 2014-06-05;327(1):68-77. 2014-06-05 [PMID: 24907654] (Flow Cytometry, Human)
• Park , Su-Jung, Kim , Ki-Jo, Kim , Wan-Uk, Cho , Chul-Soo Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol, 2014-02-26;192(7):3003-10. 2014-02-26 [PMID: 24574497] (Flow Cytometry, Human)
• Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology. J. Thromb. Haemost., 2011-05-01;9(5):1056-66. 2011-05-01 [PMID: 21342432] (Flow Cytometry, Human)
• Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis. Blood, 2010-07-06;116(16):3099-107. 2010-07-06 [PMID: 20606164] (Flow Cytometry, Human)
• Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors. Oncogene, 2010-03-08;29(20):2938-49. 2010-03-08 [PMID: 20208561] (Flow Cytometry, Human)
• Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J. Immunol., 2009-12-15;183(12):8176-85. 2009-12-15 [PMID: 20007583] (Flow Cytometry, Human)
• Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M VEGFR2 is selectively expressed by FOXP3high CD4+ Treg. Eur. J. Immunol., 2010-01-01;40(1):197-203. 2010-01-01 [PMID: 19902430] (Flow Cytometry, Human)
• Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells. J. Cell. Physiol., 2008-10-01;217(1):261-80. 2008-10-01 [PMID: 18551514] (Flow Cytometry, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation. Exp. Hematol., 2008-05-01;36(5):545-558. 2008-05-01 [PMID: 18439488] (Flow Cytometry, Human)
• Narazaki M, Segarra M, Tosato G Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 2008-02-13;111(8):4126-36. 2008-02-13 [PMID: 18272814] (Flow Cytometry, Human)
• Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression. J. Clin. Invest., 2007-11-01;117(11):3369-82. 2007-11-01 [PMID: 17948123] (Flow Cytometry, Human)
• Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007-04-30;177(3):489-500. 2007-04-30 [PMID: 17470632] (Flow Cytometry, Human)
• Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Gut, 2006-07-13;56(2):243-52. 2006-07-13 [PMID: 16840505] (Flow Cytometry, Human)
• Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC Expansion of human SCID-repopulating cells under hypoxic conditions. J. Clin. Invest., 2003-07-01;112(1):126-35. 2003-07-01 [PMID: 12840067] (Flow Cytometry, Human)

Review for VEGFR1/Flt-1 Antibody (FAB321P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/23/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 24449499
• Species: Human

Bioinformatics

• Gene Symbol: FLT1
• Uniprot:
  • NP_001153392()