VAV3 Antibody

Western Blot: VAV3 Antibody [NBP3-23274] - Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-VAV2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-VAV3 (a.a. 293-305) at 1:500 (lanes 4-6).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

VAV3 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the Dbl homology domain of human Vav3. This sequence has high homology with similar regions in rat and mouse Vav3, and has low homology to other Vav family members. [UniProt# Q9UKW4]
• Specificity: The antibody detects a 98 kDa protein corresponding to the molecular mass of Vav3 on SDS-PAGE immunoblots of human Jurkat cells. The antibody also strongly detects an unidentified protein at 120 kDa in Jurkat and K562.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VAV3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for VAV3 Antibody

• FLJ40431
• guanine nucleotide exchange factor VAV3
• vav 3 guanine nucleotide exchange factor
• vav 3 oncogene
• VAV-3

Background

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav3 is expressed in most hematopoietic cells and non-hematopoietic tissues except ovary and skeletal muscles. T-cell stimulated and tyrosine phosphorylated Vav3 acts as an exchange factor for GTP-binding proteins RhoA, RhoG, and Rac1 (2). Also, tyrosine phosphorylated Vav3 mediates RTK signalizing, specifically IR, IGFR, and EGFR-mediated signaling pathways. Vav3 has been linked to as a factor for cell morphology modulation and an inducer for cell transformation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAV3