Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Vav-1 (Y160) Monoclonal Antibody (Catalog # MAB37861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Vav-1 (Y160) at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Vav-1 phosphorylated at Y160 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Vav-1 (Y160) Monoclonal Antibody (Catalog # MAB37861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Human Phospho-Vav‑1 (Y160) at approximately 116 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Phospho-Vav‑1 (Y160) Monoclonal Antibody (Catalog # MAB37861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Applications: WB, Simple Western, ICC/IF
• Clone: 751411
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Vav-1 [p Tyr160] Antibody (751411) [Unconjugated] Summary

• Immunogen: Phosphopeptide containing the human Vav-1 Y160 site
• Modification: p Tyr160
• Specificity: Detects human VAV-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAV1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vav-1 [p Tyr160] Antibody (751411) [Unconjugated]

• oncogene vav
• proto-oncogene vav
• vav 1 guanine nucleotide exchange factor
• vav proto-oncogene
• Vav1
• Vav-1
• vav-T
• VAVvav 1 oncogene

Background

The Vav-1 proto-oncogene is a 95 kDa guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 is a cytosolic protein that is primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells. It promotes intracellular signaling by its multiple protein binding motifs. Vav-1 is phosphorylated on Y160 after cell adhesion via integrin avb3. This phosphorylation is necessary for adhesion‑mediated Rho activation and association between avb3 and Vav-1. The peptide immunogen represents a sequence that is conserved between human, mouse and rat Vav-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vav-1 Antibody (MAB37861)(1)

Publication using MAB37861

• Jenkins , Misty R, Stinchcombe , Jane C, Au-Yeung , Byron B, Asano , Yukako, Ritter , Alex T, Weiss , Arthur, Griffiths , Gillian Distinct structural and catalytic roles for Zap70 in formation of the immunological synapse in CTL. Elife, 2014-03-04;3(0):e01310. 2014-03-04 [PMID: 24596147] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: VAV1