Ribonuclease Inhibitor Antibody (OTI1B7)

Western Blot: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribonuclease Inhibitor monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ribonuclease Inhibitor.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Flow Cytometry: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Ribonuclease Inhibitor antibody, and then analyzed by flow cytometry.

Western Blot: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribonuclease Inhibitor (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribonuclease Inhibitor.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Human lymph node tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Ribonuclease Inhibitor Antibody (1B7) [NBP2-03254] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribonuclease Inhibitor mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.66 mg/ml

Ribonuclease Inhibitor Antibody (OTI1B7) Summary

• Description: Novus Biologicals Mouse Ribonuclease Inhibitor Antibody (OTI1B7) (NBP2-03254) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 49.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.66 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ribonuclease Inhibitor Antibody (OTI1B7)

• Placental ribonuclease inhibitor
• Placental RNase inhibitor
• PRI
• RAIMGC4569
• ribonuclease inhibitor
• ribonuclease/angiogenin inhibitor 1MGC18200
• ribonuclease/angiogenin inhibitor
• RNHMGC54054

Background

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNH1
• Entrez:
  • 6050(Human)