| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85792-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-16890-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82254-0.025ml | |||
![Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Western-Blot-NBP2-00555-img0011.jpg "Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-00555-img0010.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-00555-img0009.jpg)
![Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Flow-Cytometry-NBP2-00555-img0002.jpg)
![Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Western-Blot-NBP2-00555-img0003.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-00555-img0004.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-00555-img0005.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human endometrium tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-00555-img0006.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human liver tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-00555-img0007.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymph node tissue using anti-VASP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-00555-img0008.jpg)
![Flow Cytometry: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of Hela cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/VASP-Antibody-4D6-Flow-Cytometry-NBP2-00555-img0001.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Analysis in human lymph node and skeletal muscle tissues using NBP1-85792 antibody. Corresponding VASP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunohistochemistry-Paraffin-NBP1-85792-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Analysis in human lymph node and skeletal muscle tissues using NBP1-85792 antibody. Corresponding VASP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85792-img0011.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunohistochemistry-Paraffin-NBP1-85792-img0019.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Staining of human lymph node shows strong cytoplasmic positivity in lymphocytes.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunohistochemistry-Paraffin-NBP1-85792-img0016.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Staining of human stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunohistochemistry-Paraffin-NBP1-85792-img0017.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Stainin of human prostate shows moderate ctyoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunohistochemistry-Paraffin-NBP1-85792-img0018.jpg)
![Western Blot: VASP [p Ser157] Antibody [NBP3-05448] - Western blot analysis of extracts of A431 cells, using VASP antibody (NBP3-05448) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Western-Blot-NBP3-05448-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: VASP [p Ser157] Antibody [NBP3-05448] - Western blot analysis of extracts of A431 cells, using VASP antibody (NBP3-05448) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded mouse lung using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunohistochemistry-Paraffin-NBP3-05448-img0004.jpg)
![Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded rat brain using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunohistochemistry-Paraffin-NBP3-05448-img0002.jpg)
![Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded human colon using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunohistochemistry-Paraffin-NBP3-05448-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: VASP [p Ser157] Antibody [NBP3-05448] - Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug VASP pAb (NBP3-05448). Western blot was performed from the immunoprecipitate using VASP pAb (NBP3-05448) at a dilution of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37C for 30 minutes after serum-starvation overnight.](http://images.novusbio.com/fullsize/VASP-[p-Ser155]-Antibody-Immunoprecipitation-NBP3-05448-img0001.jpg)
![Western Blot: VASP [p Ser157] Antibody - Azide and BSA Free [VASP] - Western blot analysis of lysates from HEL cells using VASP Rabbit pAb at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.8s.](http://images.novusbio.com/fullsize/nbp3-05448_rabbit-polyclonal-vasp-p-ser155-antibody-5220251629390.jpg)
![Western Blot: VASP [p Ser156] Antibody (JE45-91) [NBP3-32815] - Western blot analysis of VASP on different lysates with Rabbit anti-VASP antibody (NBP3-32815) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32815) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32815_rabbit-vasp-p-ser156-mab-je45-91-288202412461679.jpg "Western Blot: VASP [p Ser156] Antibody (JE45-91) [NBP3-32815] - Western blot analysis of VASP on different lysates with Rabbit anti-VASP antibody (NBP3-32815) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 3: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate, then the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32815) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: VASP [p Ser156] Antibody (JE45-91) [NBP3-32815] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-VASP antibody (NBP3-32815) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32815) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32815_rabbit-vasp-p-ser156-mab-je45-91-298202413585518.jpg)
![Immunocytochemistry/ Immunofluorescence: VASP [p Ser156] Antibody (JE45-91) [NBP3-32815] - Immunocytochemistry analysis of A431 cells treated with or without 10μM forskolin for 1 hour labeling VASP with Rabbit anti-VASP antibody (NBP3-32815) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VASP antibody (NBP3-32815) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32815_rabbit-vasp-p-ser156-mab-je45-91-29202410234251.jpg)
![Western Blot: VASP Antibody (GT238) [NBP3-13619] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with VASP antibody [GT238] (NBP3-13619) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VASP-Antibody-GT238-Western-Blot-NBP3-13619-img0001.jpg "Western Blot: VASP Antibody (GT238) [NBP3-13619] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with VASP antibody [GT238] (NBP3-13619) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VASP Antibody (GT238) [NBP3-13619] - VASP antibody [GT238] detects VASP protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VASP stained by VASP antibody [GT238] (NBP3-13619) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/VASP-Antibody-GT238-Immunocytochemistry-Immunofluorescence-NBP3-13619-img0002.jpg)
![Immunohistochemistry-Paraffin: VASP Antibody (GT238) [NBP3-13619] - VASP antibody [GT238] detects VASP protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: VASP stained by VASP antibody [GT238] (NBP3-13619) diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody (NBP2-15569) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VASP-Antibody-GT238-Immunohistochemistry-Paraffin-NBP3-13619-img0003.jpg)
![Western Blot: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/VASP-Antibody-Western-Blot-NBP2-41363-img0002.jpg "Western Blot: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/VASP-Antibody-Western-Blot-NBP2-41363-img0001.jpg)
![Immunoprecipitation: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for IP at 6 ug per reaction. For blotting immunoprecipitated VASP, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunoprecipitation-NBP2-41363-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: VASP Antibody [NBP2-41363] - Detection of human VASP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for IP at 6 ug per reaction. VASP was also immunoprecipitated by another rabbit anti-VASP antibody. For blotting immunoprecipitated VASP, NBP2-41363 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/VASP-Antibody-Immunoprecipitation-NBP2-41363-img0004.jpg)
![Western Blot: VASP [p Ser239] Antibody [NBP3-16890] - Western blot analysis of extracts of C6 cells, using VASP antibody (NBP3-16890) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37C for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/VASP-[p-Ser239]-Antibody-Western-Blot-NBP3-16890-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: VASP [p Ser239] Antibody [NBP3-16890] - Western blot analysis of extracts of C6 cells, using VASP antibody (NBP3-16890) at 1:1000 dilution.C6 cells were treated by Forskolin (10 uM) at 37C for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: VASP [p Ser239] Antibody - Azide and BSA Free [NBP3-16890] - Immunohistochemistry analysis of paraffin-embedded Human placenta using VASP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16890_rabbit-vasp-p-ser-239-pab-52202511383778.jpg)
![Immunohistochemistry: VASP [p Ser239] Antibody - Azide and BSA Free [NBP3-16890] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using VASP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16890_rabbit-vasp-p-ser-239-pab-522025125493.jpg)
![Western Blot: VASP [p Ser238] Antibody [NB100-82254] - Analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239) Antibody](http://images.novusbio.com/fullsize/VASP-[p-Ser238]-Antibody-Western-Blot-NB100-82254-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: VASP [p Ser238] Antibody [NB100-82254] - Analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239) Antibody")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VASP [p Ser238] Antibody [NB100-82254] - Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue, using NB100-82254 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/VASP-[p-Ser238]-Antibody-Immunohistochemistry-NB100-82254-img0002.jpg)
![Immunohistochemistry-Paraffin: VASP [p Ser238] Antibody [NB100-82254] - Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue, using NB100-82254(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82254_rabbit-polyclonal-vasp-p-ser238-antibody-10320252332108.jpg)
![Immunocytochemistry/Immunofluorescence: VASP [p Thr278] Antibody [NBP3-23264] - Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. The antibody was used in the absence (top left) or presence (top right) of blocking peptide. Corresponding phase images are shown bottom left and right.](http://images.novusbio.com/fullsize/nbp3-23264_rabbit-vasp-p-thr278-pab-199202317594417.jpg "Immunocytochemistry/Immunofluorescence: VASP [p Thr278] Antibody [NBP3-23264] - Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. The antibody was used in the absence (top left) or presence (top right) of blocking peptide. Corresponding phase images are shown bottom left and right.")
![Western Blot: VASP [p Thr278] Antibody [NBP3-23264] - Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23264_rabbit-vasp-p-thr278-pab-19920231410138.jpg)
![Western Blot: VASP Antibody [NBP2-42515] - (1ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VASP-Antibody-Western-Blot-NBP2-42515-img0004.jpg "Western Blot: VASP Antibody [NBP2-42515] - (1ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: VASP Antibody [NBP2-42515] - (0.01ug/ml) staining of Human Platelets lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VASP-Antibody-Western-Blot-NBP2-42515-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: VASP Antibody (M277) [NBP3-23263] - Immunocytochemical labeling of VASP in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal VASP (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23263_mouse-vasp-mab-m277-19920231856190.jpg "Immunocytochemistry/Immunofluorescence: VASP Antibody (M277) [NBP3-23263] - Immunocytochemical labeling of VASP in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal VASP (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: VASP Antibody (M277) [NBP3-23263] - Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23263_mouse-vasp-mab-m277-19920231856910.jpg)