VASA Antibody (246904) [Unconjugated]

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VASA Monoclonal Antibody (Catalog # MAB2030) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 246904
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VASA Antibody (246904) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human VASA
  Met1-Tyr145
  Accession # Q9NQI0
• Specificity: Detects human VASA in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VASA Antibody (246904) [Unconjugated]

• DDX4
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• DEAD box protein 4
• EC 3.6.1
• EC 3.6.4.13
• MGC111074
• probable ATP-dependent RNA helicase DDX4
• Vasa homolog
• VASA
• VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

VASA, also known as DDX4, is a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is a highly specific marker of germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VASA Antibody (MAB2030)(2)

Publications using MAB2030

• Christopher Noyes, Shunsuke Kitajima, Fengkai Li, Yusuke Suita, Saradha Miriyala, Shakson Isaac et al. The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells Communications Biology 2023-01-18 [PMID: 36653474]
• Christopher Noyes, Shunsuke Kitajima, Fengkai Li, Yusuke Suita, Saradha Miriyala, Shakson Isaac et al. The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells Communications Biology 2023-01-18 [PMID: 36653474] (Immunohistochemistry, Mouse)

Bioinformatics

• Gene Symbol: DDX4
• Uniprot:
  • Q9NQI0()