VAMP-8 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) ...read more
VAMP‑8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human VAMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) at 1 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

VAMP-8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VAMP-8
Met1-Lys75
Accession # Q9BV40
Specificity
Detects human VAMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) VAMP‑1, rhVAMP‑2, rhVAMP‑5, and rhVAMP‑7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VAMP8
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 1-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5354 in the following applications:

Publications
Read Publications using
AF5354 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-8 Antibody [Unconjugated]

  • EDB
  • EDBVAMP-8
  • Endobrevin
  • VAMP8
  • VAMP-8
  • vesicle-associated membrane protein 8 (endobrevin)
  • vesicle-associated membrane protein 8

Background

VAMP-8 (vesicle associated membrane protein 8; also endobrevin) is a 12 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is predominately transmembrane) that is found in cells involved in vesicle release (platelets, mast cells and pancreatic acinar cells). It regulates exocytosis and decouples granule release from cytokine release. Human VAMP-8 is 100 amino acids (aa) in length. It contains a vSNARE coiled-coil homology region (aa 12-72) and a membrane-anchor domain (aa 76‑96). Over aa 1‑75, human VAMP-8 shares 93% aa identity with mouse
VAMP‑2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-91258
Species: Hu, Mu, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-83162
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-595
Species: Mu, Rt, Bv
Applications: WB, Flow, IHC, IHC-P, IP, IF
NB300-510
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF
MAB6117
Species: Hu
Applications: WB, IHC
NBP2-33414
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00795
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF922
Species: Hu
Applications: WB, Block
NBP1-87374
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IF
NBP1-87035
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15131
Species: Hu
Applications: WB
NBP1-87497
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB2568
Species: Mu
Applications: WB, IHC
AF5448
Species: Hu, Mu, Rt
Applications: WB, IHC
NB120-19294
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89726
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for VAMP-8 Antibody (AF5354)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species

Review for VAMP-8 Antibody (AF5354) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF5354:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot VAMP-8 AF5354
Enlarge
reviewed by:
Scott Messenger
WB Human 04/25/2017
View

Summary

ApplicationWestern Blot
Sample TestedT84 human colon carcinoma cell line
SpeciesHuman

Comments

Comments100 ug whole cell lystate 15% gel

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VAMP-8 Antibody (AF5354) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional VAMP-8 Products

Bioinformatics Tool for VAMP-8 Antibody (AF5354)

Discover related pathways, diseases and genes to VAMP-8 Antibody (AF5354). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VAMP-8 Antibody (AF5354)

Discover more about diseases related to VAMP-8 Antibody (AF5354).
 

Pathways for VAMP-8 Antibody (AF5354)

View related products by pathway.

PTMs for VAMP-8 Antibody (AF5354)

Learn more about PTMs related to VAMP-8 Antibody (AF5354).

Blogs on VAMP-8

There are no specific blogs for VAMP-8, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Scott Messenger
04/25/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol VAMP8
Entrez
Uniprot