VAMP-8 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-8 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

VAMP-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VAMP-8 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human VAMP-8
  Met1-Lys75
  Accession # Q9BV40
• Specificity: Detects human VAMP-8 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VAMP8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-8 Antibody [Unconjugated]

• EDB
• EDBVAMP-8
• Endobrevin
• VAMP8
• VAMP-8
• vesicle-associated membrane protein 8 (endobrevin)
• vesicle-associated membrane protein 8

Background

VAMP-8 (vesicle associated membrane protein 8; also endobrevin) is a 12 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is predominately transmembrane) that is found in cells involved in vesicle release (platelets, mast cells and pancreatic acinar cells). It regulates exocytosis and decouples granule release from cytokine release. Human VAMP-8 is 100 amino acids (aa) in length. It contains a vSNARE coiled-coil homology region (aa 12-72) and a membrane-anchor domain (aa 76‑96). Over aa 1‑75, human VAMP-8 shares 93% aa identity with mouse
VAMP‑2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAMP-8 Antibody (AF5354)(6)

Publications using AF5354

• S Ganesan, NN Alvarez, S Steiner, KM Fowler, AK Corona, CR Roy Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection MBio, 2023-02-02;0(0):e0354522. 2/2/2023 [PMID: 36728431]
• Ildar Gabaev, James C. Williamson, Thomas W.M. Crozier, Thomas F. Schulz, Paul J. Lehner Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation Cell Reports 10/13/2020 [PMID: 33053346]
• SM Madhavan, JF O'Toole, M Konieczkow, L Barisoni, DB Thomas, S Ganesan, LA Bruggeman, M Buck, JR Sedor APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8 JCI Insight, 2017-07-20;2(14):. 2017-07-20 [PMID: 28724794] (IHC, Human)
• Sang Su Woo, Declan J James, Thomas F J Martin Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles. Molecular Biology of the Cell, 2017-01-18;0(0):1939-4586. 2017-01-18 [PMID: 28100639] (Western Blot, Rat)
• Zhu Q, Yamakuchi M, Lowenstein C SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor. PLoS ONE, 2015-08-12;10(8):e0118737. 2015-08-12 [PMID: 26266817] (IHC, Western Blot, Human)
• Henri-François Renard, Maria Daniela Garcia-Castillo, Valérie Chambon, Christophe Lamaze, Ludger Johannes Shiga toxin stimulates clathrin-independent endocytosis of the VAMP2, VAMP3 and VAMP8 SNARE proteins Journal of Cell Science 8/1/2015 [PMID: 26071526]

Review for VAMP-8 Antibody (AF5354) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Scott Messenger WB Human 04/25/2017

Summary

• Application: Western Blot
• Sample Tested: T84 human colon carcinoma cell line
• Species: Human

Comments

• Comments: 100 ug whole cell lystate 15% gel

Bioinformatics

• Gene Symbol: VAMP8
• Uniprot:
  • Q9BV40()