VAMP-8 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) ...read more
VAMP‑8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human VAMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) at 1 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

VAMP-8 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human VAMP-8
Met1-Lys75
Accession # Q9BV40
Specificity
Detects human VAMP-8 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VAMP8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5354 in the following applications:

Publications
Read Publications using
AF5354 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-8 Antibody [Unconjugated]

  • EDB
  • EDBVAMP-8
  • Endobrevin
  • VAMP8
  • VAMP-8
  • vesicle-associated membrane protein 8 (endobrevin)
  • vesicle-associated membrane protein 8

Background

VAMP-8 (vesicle associated membrane protein 8; also endobrevin) is a 12 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is predominately transmembrane) that is found in cells involved in vesicle release (platelets, mast cells and pancreatic acinar cells). It regulates exocytosis and decouples granule release from cytokine release. Human VAMP-8 is 100 amino acids (aa) in length. It contains a vSNARE coiled-coil homology region (aa 12-72) and a membrane-anchor domain (aa 76‑96). Over aa 1‑75, human VAMP-8 shares 93% aa identity with mouse
VAMP‑2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC

Publications for VAMP-8 Antibody (AF5354)(3)

Review for VAMP-8 Antibody (AF5354) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF5354:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot VAMP-8 AF5354
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5
reviewed by:
Verified Customer
WB Human 04/25/2017
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Summary

ApplicationWestern Blot
Sample TestedT84 human colon carcinoma cell line
SpeciesHuman

Comments

Comments100 ug whole cell lystate 15% gel

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
04/25/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol VAMP8
Uniprot