VAMP-1 Antibody

Images

 
Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse VAMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) followed by ...read more
VAMP‑1 was detected in perfusion fixed frozen sections of mouse spinal cord using 1.7 µg/mL Goat Anti-Human/Mouse VAMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

VAMP-1 Antibody Summary

Immunogen
E. coli-derived recombinant human VAMP-1
Met1-Lys96
Accession # P23763
Specificity
Detects human and mouse VAMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VAMP-2 and less than 1% cross-reactivity with rhVAMP-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VAMP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF4828 in the following applications:

Publications
Read Publication using
AF4828 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-1 Antibody

  • DKFZp686H12131
  • SYB1
  • SYB1vesicle-associated membrane protein 1
  • synaptobrevin 1
  • Synaptobrevin-1
  • VAMP1
  • VAMP-1
  • VAMP-1synaptobrevin-1
  • vesicle-associated membrane protein 1 (synaptobrevin 1)

Background

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is 118 amino acids (aa) in length. It is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1-96) and a 22 aa transmembrane domain (aa 97-118). There is one coiled-coil region between aa 33-93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1-96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAMP-1 Antibody (AF4828)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for VAMP-1 Antibody (AF4828) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF4828:
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WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Rat 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23206873
SpeciesRat

Blocking

Blocking DetailsSee PMID 23206873

Details

Detection NotesSee PMID 23206873

Comments

CommentsPublished: See PMID 23206873Independent review from antibodyresource.com

Product General Protocols

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Video Protocols

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FAQs for VAMP-1 Antibody (AF4828) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for VAMP-1 Antibody (AF4828)

Discover related pathways, diseases and genes to VAMP-1 Antibody (AF4828). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
1
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2
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1
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Anonymous
01/08/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol VAMP1
Entrez
Uniprot