VAMP-2 Antibody

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Western Blot: VAMP-2 Antibody [NB300-595] - Analysis was performed on tissues (40 ug lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2) and Rat Brain (Lane 3).
Flow Cytometry: VAMP-2 Antibody [NB300-595] - Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.
Western Blot: VAMP-2 Antibody [NB300-595] - Analysis of rat brain extract.

Product Details

Summary
Reactivity Mu, Rt, Bv, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

VAMP-2 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2.
Localization
Type IV membrane protein. Neuronal synaptic vesicles
Marker
Synaptic Vesicles Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VAMP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 ug/ml
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunofluorescence 1:10 - 1:500
Application Notes
IHC usage was reported in scientific literature (See Windoffer R, et al. 1999). WB: Detects an approx. 19 kDa protein representing VAMP 2 from rat brain whole protein extract.
Publications
Read Publications using NB300-595.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 10370136).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAMP-2 Antibody

  • FLJ11460
  • SYB2
  • SYB2vesicle-associated membrane protein 2
  • synaptobrevin 2
  • synaptobrevin-2
  • VAMP2
  • VAMP-2
  • VAMP-2vesicle associated membrane protein 2
  • vesicle-associated membrane protein 2 (synaptobrevin 2)

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP 25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP 2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VAMP2
Entrez