VAChT/SLC18A3 Antibody (HL2900)

VAChT/SLC18A3 antibody [HL2900] detects VAChT/SLC18A3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VAChT/SLC18A3 stained by VAChT/SLC18A3 antibody [HL2900] (NBP3-48748) diluted at 1:100.Red: beta Tubulin 3/ TUJ1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

VAChT/SLC18A3 antibody [HL2900] detects VAChT/SLC18A3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: VAChT/SLC18A3 stained by VAChT/SLC18A3 antibody [HL2900] (NBP3-48748) diluted at 1:100.Red: beta Tubulin 3/ TUJ1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Mu, Rt
• Applications: IHC
• Clone: HL2900
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

VAChT/SLC18A3 Antibody (HL2900) Summary

• Description: Novus Biologicals Rabbit VAChT/SLC18A3 Antibody (HL2900) (NBP3-48748) is a recombinant monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the Intracellular domain of human VAChT/SLC18A3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLC18A3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for VAChT/SLC18A3 Antibody (HL2900)

• MGC12716
• SLC18A3
• solute carrier family 18 (vesicular acetylcholine), member 3
• Solute carrier family 18 member 3
• solute carrier family 18, member 3
• VAChT
• VACHTvesicular acetylcholine transporter

Background

Vesicular Acetylcholine Transporter (VAChT), (approx. 70kD protein), belongs to the family of vesicular monoamine transporters(VMATs), which include VMAT1 and VMAT2 and the C.elegans putative ACh transporter unc-17. Members of this family function to concentrate neurotransmitters into synaptic vesicles through exchange of protons for neurotransmitters. VAChT is a functional transporter for the neurotransmitter acetylcholine(ACh). ACh is synthesized in the cytoplasm by choline acetyl transferase (ChAT) and transported by VAChT into synaptic vesicles where it is stored until released. After release from presynaptic nerve terminals ACh is hydrolyzed by extracellular ACh-esterases(AChE) to choline and acetate. VAChT mRNA is expressed in all known major cholinergic neurons in the central and peripheral nervous system. VAChT is abundantly expressed in the CNS and is mainly localized in small synaptic vesicles in cholinergic nerve terminals. VAChT provides a specific marker for cholinergic neurons for the study of cholinergic transmission in experimental models, in Alzheimer's disease and other nervous system disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC18A3