UVRAG Antibody (953035) [Unconjugated]

Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Monoclonal Antibody (Catalog # MAB9180, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human UVRAG Monoclonal Antibody (Catalog # MAB9180, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 953035
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

UVRAG Antibody (953035) [Unconjugated] Summary

• Immunogen: Human UVRAG synthetic peptide corresponding to sequences between amino acids 260-360
  Accession # Q9P2Y5
• Specificity: Detects human UVRAG in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UVRAG Antibody (953035) [Unconjugated]

• beclin 1 binding protein
• DHTX
• disrupted in heterotaxy
• p63
• UV radiation resistance associated gene
• UV radiation resistance-associated gene protein
• UVRAG
• VPS38

Background

UV radiation resistance-associated gene protein, or UVRAG, is a multi-function protein of 699 amino acids involved in the regulation of cellular pathways implicated in membrane trafficking. UVRAG activates the Beclin-1-PI3KC3 complex, a critical element in the autophagy signaling pathway. Binding studies show that UVRAG and Beclin-1 directly interact via their coiled-coil domains and that Beclin-1 functions as a platform for the formation of the Bcl-2-UVRAG-PI3KC3 complex. Chromosomal aberrations involving the UVRAG gene are associated with left-right axis malformation, and mutations in the gene have been associated with colon cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9P2Y5()