USP7 Antibody (6512) - Azide and BSA Free

Western Blot: USP7 Antibody (6512) [NBP2-43619] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 5 % SDS-PAGE USP7 antibody [6512] dilution: 1:1000

USP7 antibody [GT6512] detects USP7 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: USP7 protein stained by USP7 antibody [GT6512] (NBP2-43619) diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Po, Ch, Xp
• Applications: WB, ICC/IF, IP
• Clone: 6512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

USP7 Antibody (6512) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse USP7 Antibody (6512) - Azide and BSA Free (NBP2-43619) is a monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (98%), Porcine (97%), Chicken (93%), Xenopus (93%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 128 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (93%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for USP7 Antibody (6512) - Azide and BSA Free

• Deubiquitinating enzyme 7
• EC 3.1.2.15
• EC 3.4.19.12
• HAUSP
• HAUSPTEF1
• Herpes virus-associated ubiquitin-specific protease
• Herpesvirus-associated ubiquitin-specific protease
• TEF1
• ubiquitin carboxyl-terminal hydrolase 7
• ubiquitin specific peptidase 7 (herpes virus-associated)
• ubiquitin specific protease 7 (herpes virus-associated)
• Ubiquitin thioesterase 7
• Ubiquitin-specific-processing protease 7
• USP7

Background

HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity-purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53 even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP7