USP4 Antibody

Immunohistochemistry-Paraffin: USP4 Antibody [NB100-90597] - Anti-USP4 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: USP4 Antibody [NB100-90597] - Analysis of anti-USP4 / UNP antibody with human lung, non-small cell carcinoma.

Immunohistochemistry-Paraffin: USP4 Antibody [NB100-90597] - Anti-USP4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Discontinued

USP4 Antibody Summary

• Immunogen: Synthetic 19 amino acid peptide from Near the N-terminus of human USP4.
• Epitope: Near the N-terminus of human
• Specificity: Human USP4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Orangutan (95%), Rabbit (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:1000 - 1:10000
• Control:

  USP4 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (95%), Gibbon (89%), Marmoset (89%), Canine (89%), Bovine (89%), Equine (89%), Monkey (84%), Opossum (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP4 Antibody

• Deubiquitinating enzyme 4
• EC 3.1.2.15
• EC 3.4.19.12
• MGC149848
• MGC149849
• ubiquitin carboxyl-terminal esterase 4
• ubiquitin carboxyl-terminal hydrolase 4
• ubiquitin specific peptidase 4 (proto-oncogene)
• ubiquitin specific protease 4 (proto-oncogene)
• ubiquitin thioesterase 4
• Ubiquitin thiolesterase 4
• ubiquitin-specific processing protease 4
• Ubiquitin-specific-processing protease 4
• Ubiquitous nuclear protein homolog
• UNP
• Unph
• UNPUnph
• USP4

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP4
• Entrez:
  • 7375(Human)
• Uniprot:
  • Q13107()