USP33 Antibody (5B5)

Images

 
Western Blot: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.Lane 1: USP33 transfected lysate(103.3 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: USP33 Antibody (5B5) [H00023032-M01] - USP33 expression in HeLa ( Cat # L013V1 ).
Immunoprecipitation: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 MaxPab rabbit polyclonal ...read more
Sandwich ELISA: USP33 Antibody (5B5) [H00023032-M01] - Detection limit for recombinant GST tagged USP33 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
5B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

USP33 Antibody (5B5) Summary

Immunogen
USP33 (NP_055832, 327 a.a. ~ 427 a.a) partial recombinant protein with GST tag.SDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKM CNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASE YITDVHSNDLSTPQIL
Localization
Cytoplasmic
Specificity
USP33 - ubiquitin specific protease 33
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
USP33
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP33 Antibody (5B5)

  • Deubiquitinating enzyme 33
  • EC 3.1.2.15
  • EC 3.4.19.12
  • hVDU1
  • KIAA1097MGC16868
  • pVHL-interacting deubiquitinating enzyme 1
  • ubiquitin carboxyl-terminal hydrolase 33
  • ubiquitin specific peptidase 33
  • ubiquitin thioesterase 33
  • Ubiquitin thiolesterase 33
  • Ubiquitin-specific-processing protease 33
  • VDU1ubiquitin specific protease 33
  • VHL-interacting deubiquitinating enzyme 1

Background

The ubiquitin dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitination, is catalyzed by proteases called deubiquitinating enzymes. USP33 is a deubiquitinating enzyme that binds to the von Hippel-Lindau tumor suppressor protein. It also regulates thyroid hormone activation by deubiquitinating type 2 iodothyronine deiodinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP33
Entrez
Uniprot