USP33 Antibody (5B5) - Azide and BSA Free

Western Blot: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.Lane 1: USP33 transfected lysate(103.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on HeLa cell. Antibody concentration 20 ug/ml.

Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Western Blot: USP33 Antibody (5B5) [H00023032-M01] - USP33 expression in HeLa ( Cat # L013V1 ).

Immunoprecipitation: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: USP33 Antibody (5B5) [H00023032-M01] - Detection limit for recombinant GST tagged USP33 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 5B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

USP33 Antibody (5B5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse USP33 Antibody (5B5) - Azide and BSA Free (H00023032-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: USP33 (NP_055832, 327 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQIL
• Localization: Cytoplasmic
• Specificity: USP33 - ubiquitin specific protease 33
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP33
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP33 Antibody (5B5) - Azide and BSA Free

• Deubiquitinating enzyme 33
• EC 3.1.2.15
• EC 3.4.19.12
• hVDU1
• KIAA1097
• KIAA1097MGC16868
• pVHL-interacting deubiquitinating enzyme 1
• ubiquitin carboxyl-terminal hydrolase 33
• ubiquitin specific peptidase 33
• ubiquitin thioesterase 33
• Ubiquitin thiolesterase 33
• Ubiquitin-specific-processing protease 33
• USP33
• VDU1
• VDU1ubiquitin specific protease 33
• VHL-interacting deubiquitinating enzyme 1

Background

The ubiquitin dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitination, is catalyzed by proteases called deubiquitinating enzymes. USP33 is a deubiquitinating enzyme that binds to the von Hippel-Lindau tumor suppressor protein. It also regulates thyroid hormone activation by deubiquitinating type 2 iodothyronine deiodinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP33
• Entrez:
  • 23032(Human)
• Uniprot:
  • NP_055832(Human)