USP22 Antibody

Images

 
Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.
Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified ...read more
Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

USP22 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human USP22 protein (within residues 100-205). [Swiss-Prot Q9H3R0]
Localization
Nucleus.
Clonality
Polyclonal
Host
Rabbit
Gene
USP22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:25
  • Immunohistochemistry-Paraffin
Application Notes
This Usp22 antibody is useful for Western blot, where a band is seen ~59 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24197134).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49644 in the following applications:

Publications
Read Publications using
NBP1-49644 in the following applications:

  • 1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for USP22 Antibody

  • Deubiquitinating enzyme 22
  • EC 3.1.2.15
  • EC 3.4.19.12
  • KIAA1063ubiquitin carboxyl-terminal hydrolase 22
  • KIAA1064
  • ubiquitin specific peptidase 22
  • ubiquitin specific peptidase 3-like
  • ubiquitin specific protease 22
  • Ubiquitin Thioesterase 22
  • Ubiquitin thiolesterase 22
  • ubiquitin-specific processing protease 22
  • Ubiquitin-specific-processing protease 22
  • USP22
  • USP3L

Background

USP22 is the component of the histone acetylation complex, SAGA, that acts as a deubiquinating enzyme. It is a cofactor for gene promoters like MYC. Depleting USP22 prevents MYC from controlling the transcription and expression of thousands of other genes. Therefore, it can be postulated that USP22 is the critical mediator of MYC induced oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP22 Antibody (NBP1-49644)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(2)
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Filter By Species
Human
(2)
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Review for USP22 Antibody (NBP1-49644) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49644:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot USP22 NBP1-49644
Enlarge
reviewed by:
Anonymous
WB Human 11/25/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
LotA-1
Special ApplicationsHeLa Whole cell lysate, Antibody dilution used is 1:500 overnight 4 degree
FileView PDF

Product General Protocols

View specific protocols for USP22 Antibody (NBP1-49644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for USP22 Antibody (NBP1-49644) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49644AF405
Alexa Fluor 488 Labeled NBP1-49644AF488
Alexa Fluor 647 Labeled NBP1-49644AF647
Alexa Fluor 700 Labeled NBP1-49644AF700
Biotin Labeled NBP1-49644B
DyLight 405 Labeled NBP1-49644V
DyLight 488 Labeled NBP1-49644G
DyLight 550 Labeled NBP1-49644R
DyLight 650 Labeled NBP1-49644C
FITC Labeled NBP1-49644F
HRP Labeled NBP1-49644H

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Contact Information

Product PDFs

Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
11/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol USP22
Entrez
Uniprot

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