![Immunocytochemistry/Immunofluorescence: ENY2 Antibody (245) [NBP2-43547] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: ENY2 protein stained by ENY2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ENY2-Antibody-245-Immunocytochemistry-Immunofluorescence-NBP2-43547-img0001.jpg "Immunocytochemistry/Immunofluorescence: ENY2 Antibody (245) [NBP2-43547] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: ENY2 protein stained by ENY2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
| Reactivity | Hu, Rt, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 245 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Mouse ENY2 Antibody (245) - Azide and BSA Free (NBP2-43547) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ENY2. The exact sequence is proprietary. |
| Predicted Species | Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENY2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ENY2 |
![Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (NBP2-43547) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43547_mouse-monoclonal-eny2-antibody-245-western-blot-261120251829018.jpg "Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (NBP2-43547) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (NBP2-43547) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43547_mouse-monoclonal-eny2-antibody-245-western-blot-261120251824393.jpg "Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 antibody [GT245] (NBP2-43547) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 antibody [GT245] (NBP2-43547) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43547_mouse-monoclonal-eny2-antibody-245-immunohistochemistry-paraffin-2611202518202321.jpg "ENY2 antibody [GT245] detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 antibody [GT245] (NBP2-43547) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")