USP16 Antibody

Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for IP at 3 mcg/mg lysate. USP16 was also immunoprecipitated by rabbit anti- USP16 antibody NB100-81639, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IP, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

USP16 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human ubiquitin specific peptidase 16 using the numbering given in entry NP_006438.1 (GeneID 10600).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
• Application Notes: This antibody is not recommended for Western Blot analysis.
• Control:

  USP16 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP16 Antibody

• Deubiquitinating enzyme 16
• EC 3.1.2.15
• EC 3.4.19.12
• human ubiquitin processing protease, EC 3.1.2.1510ubiquitin specific protease 16
• ubiquitin carboxyl-terminal hydrolase 16
• ubiquitin specific peptidase 16
• ubiquitin thioesterase 16
• Ubiquitin thiolesterase 16
• Ubiquitin-processing protease UBP-M
• ubiquitin-specific processing protease 16
• Ubiquitin-specific-processing protease 16
• UBP-M
• USP16

Background

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP16
• Entrez:
  • 10600(Human)
• Uniprot:
  • NP_006438.1(Human)