Uroplakin III Antibody

Western Blot: Uroplakin III Antibody [NBP2-29477] - Human HEK293 lysate in the 1) absence and 2) presence of immunizing peptide using Uroplakin III antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Uroplakin III Antibody Summary

• Description: 200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33126)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A synthetic peptide, aa260-279 (GASESSYTSVNRGPPLDRAE) of human Uroplakin III (Uniprot: O75631)
• Localization: Cell surface
• Marker: Bladder Carcinoma Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UPK3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The amino acid sequence used as the immunogen for this UPK3A antibody is 100% homologous in human and 85% in mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Uroplakin III Antibody

• MGC119178
• UP3a
• UPIII
• UPIIIA
• UPK3
• UPK3A
• uroplakin 3
• uroplakin 3A
• Uroplakin III
• uroplakin3a
• uroplakin-3a

Background

UPK3A, is an abbreviation for Uroplakin-3A, also called UP3a and UPIIIa, is a component of the asymmetric unit membrane (AUM) that is a highly specialized biomembrane made by terminally differentiated urothelial cells. The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. UPK3A also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection. FimH initiates bladder pathology by binding to the UPK3A uroplakin receptor complex, but the subsequent events mediating pathogenesis have not been fully characterized. Bacteria-induced UPK3a signaling may mediate bladder responses to insult by uropathogenic E. coli or UPEC. Besides structure and function, UPK3A also may be a tumor cell marker inversely expressed with malignancy grade of bladder cancer and it has already been shown as a urinary marker for the detection of bladder cancer. Antibody to UPK3A is a useful tool for characterizing the anti-apoptotic, structural and functional activities of this molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Uroplakin III Antibody (NBP2-29477). (Showing 1 - 1 of 1 FAQ).

1. Would you be able to share the recommended working concentration in WB for NBP2-29477 - Uroplakin III Antibody?
   • The recommended working dilution for western blot is 2-5ug/ml

Bioinformatics

• Gene Symbol: UPK3A
• Uniprot:
  • O75631(Human)