UNC13D/Munc 13-4 Antibody (1224B) [Unconjugated]

Western blot shows lysates of NK-92 human natural killer lymphoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse UNC13D Monoclonal Antibody (Catalog # MAB89662) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for UNC13D at approximately 95-115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, CH‑1 mouse B cell lymphoma cell line, and CTLL‑2 mouse cytotoxic T cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112-122 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse UNC13D Monoclonal Antibody (Catalog # MAB89662) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western
• Clone: 1224B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

UNC13D/Munc 13-4 Antibody (1224B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: KLH-coupled N-terminal human UNC13D peptide
  Accession # Q70J99
• Specificity: Detects human and mouse UNC13D in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UNC13D
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC13D/Munc 13-4 Antibody (1224B) [Unconjugated]

• FHL3
• HLH3
• HPLH3
• Munc13-4
• Munc13-4protein unc-13 homolog D
• unc-13 homolog D (C. elegans)
• UNC13D

Background

UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues.  UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acids (aa) 40-1090 but have distinct N-terminal segments.  Human and mouse have 75% aa sequence identity throughout the region used as the immunogen.  It is unknown whether mice express the alternative isoform. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for UNC13D/Munc 13-4 Antibody (MAB89662). (Showing 1 - 1 of 1 FAQ).

1. I'm looking Munc13-4 N terminal antibody with related epitope sequence.
   • Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.

Bioinformatics

• Gene Symbol: UNC13D
• Uniprot:
  • Q70J99()