UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

Western Blot: UFM1 Activating Enzyme/UBA5 Antibody [NBP3-35568] - Western blot analysis of various lysates using UFM1 Activating Enzyme/UBA5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.

Immunohistochemistry: UFM1 Activating Enzyme/UBA5 Antibody [NBP3-35568] - Immunohistochemistry analysis of paraffin-embedded Rat liver using UFM1 Activating Enzyme/UBA5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UFM1 Activating Enzyme/UBA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UFM1 Activating Enzyme/UBA5 Antibody - BSA Free (NBP3-35568) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human UFM1 Activating Enzyme/UBA5 (NP_079094.1).
  
  Sequence:
  MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:200 - 1:2000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

• FLJ17281
• FLJ23251UBA5
• THIFP1
• UBA5
• UBE1DC1
• UBE1DC1ubiquitin-activating enzyme E1 homolog
• ubiquitin-like modifier activating enzyme 5
• ubiquitin-like modifier-activating enzyme 5
• UFM1 Activating Enzyme
• UFM1-activating enzyme

Background

UBA5 encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA5