Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF Summary

Details of Functionality
Recombinant Human Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.


  • Enzyme Activity
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
E-305 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF

  • A1S9T and BN75 temperature sensitivity complementing
  • A1S9T
  • A1ST
  • AMCX1
  • GXP1
  • MGC4781
  • POC20 centriolar protein homolog
  • POC20
  • Protein A1S9
  • SMAX2
  • UBA1
  • UBA1, ubiquitin-activating enzyme E1 homolog A
  • UBA1A
  • UBE1
  • UBE1A1S9
  • UBE1X
  • ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
  • Ubiquitin-activating enzyme E1
  • Ubiquitinactivating Enzyme
  • Ubiquitin-activating Enzyme
  • ubiquitin-like modifier activating enzyme 1
  • ubiquitin-like modifier-activating enzyme 1


Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.   

  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.

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Publications for Ubiquitin-activating Enzyme/UBE1 (E-305)(103)

We have publications tested in 10 confirmed species: Human, Mouse, Amoeba - Entamoeba histolytica, Bacteria - E. Coli, Complex Species Category, Escherichia coli, N/A, Spodoptera frugiperda, Virus - HPV, Worm - Spodoptera frugiperda (Fall Army Worm).

We have publications tested in 4 applications: Bioassay, Enzyme Assay, Ubiquitination, Ubiquitylation.

Filter By Application
Enzyme Assay
All Applications
Filter By Species
Amoeba - Entamoeba histolytica
Bacteria - E. Coli
Complex Species Category
Escherichia coli
Spodoptera frugiperda
Virus - HPV
Worm - Spodoptera frugiperda (Fall Army Worm)
All Species
Showing Publications 1 - 10 of 103. Show All 103 Publications.
Publications using E-305 Applications Species
Y Zhou, R Zheng, S Liu, C Disoma, A Du, S Li, Z Chen, Z Dong, Y Zhang, S Li, P Liu, A Razzaq, X Chen, Y Liao, S Tao, Y Liu, L Xu, Q Zhang, J Peng, X Deng, S Li, T Jiang, Z Xia Host E3 ligase HUWE1 attenuates the pro-apoptotic activity of the MERS-CoV accessory protein ORF3 by promoting its ubiquitin-dependent degradation The Journal of Biological Chemistry, 2022;0(0):101584. 2022 [PMID: 35032548] (Bioassay, Human) Bioassay Human
S Yoon, K Bogdanov, D Wallach Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 34999730] (Bioassay, Human) Bioassay Human
M Won, KA Park, S Kim, E Ju, Y Ko, H Yoo, H Ro, J Lee, J Oh, EG Lee, SY Kim, SW Nam, HM Shen, MK Yeo, JM Kim, GM Hur ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-kappaB Cell Death and Differentiation, 2021;0(0):. 2021 [PMID: 34839354] (Bioassay, Human) Bioassay Human
T Mahlokozer, B Patel, H Chen, P Desouza, X Qu, DD Mao, D Hafez, W Yang, R Taiwo, M Paturu, A Salehi, AD Gujar, GP Dunn, N Mosammapar, AA Petti, H Yano, AH Kim Competitive binding of E3 ligases TRIM26 and WWP2 controls SOX2 in glioblastoma Nature Communications, 2021;12(1):6321. 2021 [PMID: 34732716] (Bioassay, Human) Bioassay Human
RK Manne, Y Agrawal, SK Malonia, S Banday, S Edachery, A Patel, A Kumar, P Shetty, MK Santra FBXL20 promotes breast cancer malignancy by inhibiting apoptosis through degradation of PUMA and BAX The Journal of Biological Chemistry, 2021;0(0):101253. 2021 [PMID: 34587475] (Bioassay, Human) Bioassay Human
Y Liu, C Qin, Y Rao, C Ngo, JJ Feng, J Zhao, S Zhang, TY Wang, J Carriere, AC Savas, M Zarinfar, S Rice, H Yang, W Yuan, JA Camarero, J Yu, XS Chen, C Zhang, P Feng SARS-CoV-2 Nsp5 Demonstrates Two Distinct Mechanisms Targeting RIG-I and MAVS To Evade the Innate Immune Response MBio, 2021;0(0):e0233521. 2021 [PMID: 34544279] (Bioassay, Escherichia coli) Bioassay Escherichia coli
G Kokic, FR Wagner, A Chernev, H Urlaub, P Cramer Structural basis of human transcription-DNA repair coupling Nature, 2021;0(0):. 2021 [PMID: 34526721] (Bioassay, Human) Bioassay Human
C Shen, A Nayak, LR Neitzel, AA Adams, M Silver-Ise, LM Sawyer, H Benchabane, H Wang, N Bunnag, B Li, DT Wynn, F Yang, M Garcia-Con, CH Williams, S Dakshanamu, CC Hong, NG Ayad, AJ Capobianco, Y Ahmed, E Lee, DJ Robbins The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling Nature Communications, 2021;12(1):5263. 2021 [PMID: 34489457] (Bioassay, Human) Bioassay Human
Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation Nature Communications, 2021;12(1):5010. 2021 [PMID: 34408146] (Bioassay) Bioassay
CW Davies, SE Vidal, L Phu, J Sudhamsu, TB Hinkle, S Chan Rosen, FR Schumacher, YJ Zeng, C Schwerdtfe, AS Peterson, JR Lill, CM Rose, AS Shaw, IE Wertz, DS Kirkpatric, JT Koerber Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W Nature Communications, 2021;12(1):4608. 2021 [PMID: 34326324] (Bioassay, Human) Bioassay Human
Show All 103 Publications.

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Gene Symbol UBA1