CDK5RAP3 Antibody (1G4)

Western Blot: CDK5RAP3 Antibody (1G4) [H00080279-M01] - CDK5RAP3 monoclonal antibody (M01), clone 1G4 Analysis of CDK5RAP3 expression in A-431.

Immunocytochemistry/Immunofluorescence: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Analysis of monoclonal antibody to CDK5RAP3 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Analysis of monoclonal antibody to CDK5RAP3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Sandwich ELISA: CDK5RAP3 Antibody (1G4) [H00080279-M01] - Detection limit for recombinant GST tagged CDK5RAP3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CDK5RAP3 Antibody (1G4) Summary

• Immunogen: CDK5RAP3 (AAH09957, 1 a.a. ~ 506 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDHQHVPIDIQTSKLLDWLVDRRHCSLKWQSLVLTIREKINAAIQDMPESEEIAQLLSGSYIHYFHCLRILDLLKGTEASTKNIFGRYSSQRMKDWQEIIALYEKDNTYLVELSSLLVRNVNYEIPSLKKQIAKCQQLQQEYSRKEEECQAGAAEMREQFYHSCKQYGITGENVRGELLALVKDLPSQLAEIGAAAQQSLGEAIDVYQASVGFVCESPTEQVLPMLRFVQKRGNSTVYEWRTGTEPSVVERPHLEELPEQVAEDAIDWGDFGVEAVSEGTDSGISAEAAGIDWGIFPESDSKDPGGDGIDWGDDAVALQITVLEAGTQAPEGVARGPDALTLLEYTETRNQFLDELMELEIFLAQRAVELSEEADVLSVSQFQLAPAILQGQTKEKMVTMVSVLEDLIGKLTSLQLQHLFMILASPRYVDRVTEFLQQKLKQSQLLALKKELMVQKQQEALEEQAALEPKLDLLLEKTKELQKLIEADISKRYSGRPVNLMGTSL
• Specificity: CDK5RAP3 - CDK5 regulatory subunit associated protein 3
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDK5RAP3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDK5RAP3 Antibody (1G4)

• C53
• CDK5 activator-binding protein C53
• CDK5 regulatory subunit associated protein 3
• FLJ13660
• HSF-27
• IC53CDK5 regulatory subunit-associated protein 3
• ischemic heart CDK5 activator-binding protein C53
• LXXLL/leucine-zipper-containing ARFbinding protein
• LZAP
• MST016
• OK/SW-cl.114
• Protein HSF-27

Background

Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein, which may be a substrate of neuronal CDC2-like kinase, has also been found in vascular endothelial cells, where it mediates cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDK5RAP3 Antibody (H00080279-M01)(4)

Publications using H00080279-M01

• Ishimura R, El-Gowily AH, Noshiro D et al. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3 Nature communications 2022-12-21 [PMID: 36543799] (WB, Human)
  
  Details:
  Dilution used in WB 1:500
• Ishimura R, El-Gowily AH, Noshiro D et al. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3 Nature communications 2022-12-21 [PMID: 36543799] (WB, Human)
  
  Details:
  Dilution used in WB 1:500
• Ishimura R, Ito S, Mao G et al. Mechanistic insights into the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control bioRxiv 2023-02-17 (WB, Human)
• Shiwaku H, Yoshimura N, Tamura T et al. Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J 29(14):2446-60. 2010-07-21 [PMID: 20531390]

Bioinformatics

• Gene Symbol: CDK5RAP3
• Entrez:
  • 80279(Human)
• OMIM:
  • 608202(Human)
• Uniprot:
  • AAH09957(Human)