UCP3 Antibody (6B8C6) - BSA Free

Western Blot: UCP3 Antibody (6B8C6) [NBP2-61821] - Analysis using UCP3 mAb against human UCP3 (AA: 1-113 and 217-312) recombinant protein. (Expected MW is 24 kDa)

Immunocytochemistry/Immunofluorescence: UCP3 Antibody (6B8C6) [NBP2-61821] - Analysis of HL-7702 cells using UCP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Western Blot: UCP3 Antibody (6B8C6) [NBP2-61821] - Analysis using UCP3 mAb against HEK293 (1) and UCP3 (AA:1-113 and 217-312)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: UCP3 Antibody (6B8C6) [NBP2-61821] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 6B8C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

UCP3 Antibody (6B8C6) - BSA Free Summary

• Description: Novus Biologicals Mouse UCP3 Antibody (6B8C6) - BSA Free (NBP2-61821) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human UCP3 (AA: 1-113 and 217-312) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: UCP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 34.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for UCP3 Antibody (6B8C6) - BSA Free

• mitochondrial uncoupling protein 3
• SLC25A9Solute carrier family 25 member 9
• UCP 3
• uncoupling protein 3 (mitochondrial, proton carrier)
• Uncoupling protein-3

Background

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCP3