UCH-L1/PGP9.5 Antibody

Product Discontinued

UCH-L1/PGP9.5 Antibody Summary

• Immunogen: Synthetic peptide NFRKKQIEELKGQEVSPK corresponding to amino acid sequence 46 - 63 of human PGP9.5.
• Marker: pan-Neuronal Marker
• Specificity: NB600-842 recognizes protein gene product 9.5 (also known as Ubiquitin Carboxyl - Terminal Hydrolase L1). PGP9.5 is located primarily in the cytoplasm of neurons and represents 1-2% of total soluble brain proteins. PGP9.5 is involved in the processing of ubiquitin precursors and ubiquinated proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UCHL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot detects a band of approximately 27 kDa.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: 0.09% Sodium Azide
• Purity: IgG purified

Alternate Names for UCH-L1/PGP9.5 Antibody

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCHL1
• Entrez:
  • 7345(Human)
• Uniprot:
  • P09936(Human)