UCH-L1/PGP9.5 Antibody

Immunohistochemistry: UCH-L1/PGP9.5 Antibody [NB100-1640] - PGP9.5 / UCHL-1 Antibody [NB100-1640] - Staining of the synovium from ASIC3 +/+ mice with joint inflammation. Bar = 25 um. doi: 0.1016/j.pain.2008.01.020.

Immunohistochemistry: PGP9.5 / UCHL-1 Antibody [NB100-1640] - Rat Submuccus Plexus

Product Discontinued

UCH-L1/PGP9.5 Antibody Summary

• Immunogen: Synthetic peptide: GASSEDTLLKDAAKVCR, corresponding to amino acids 175-191 of UCHL1
• Localization: Cytoplasmic
• Marker: pan-Neuronal Marker
• Specificity: PGP9.5
• Isotype: IgG
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: UCHL1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot
• Application Notes: Use in western blot reported in scientific literature (PMID 27388773)
• Control:

  UCH-L1/PGP9.5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for UCH-L1/PGP9.5 Antibody

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

PGP9.5 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. It is present in neurons and in cells of the diffuse neuroendocrine system. PGP9.5 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. This enzyme is also known as UCH-L1. Because of its abundance in nerves, it has been widely used as a marker for peripheral nerve fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCH-L1/PGP9.5 Antibody (NB100-1640)(3)

Publications using NB100-1640

• Melo CGS, Nicolai EN, Alcaino C et al. Identification of intrinsic primary afferent neurons in mouse jejunum Neurogastroenterol Motil 2020-09-28 [PMID: 32986284]
• Rein S, Esplugas M, Garcia-Elias M, et al. Immunofluorescence analysis of sensory nerve endings in the interosseous membrane of the forearm J. Anat. 2019-12-20 [PMID: 31863467]
• Takahashi N, Matsuda Y, Sato K et al. Neuronal TRPV1 activation regulates alveolar bone resorption by suppressing osteoclastogenesis via CGRP Sci Rep 2016-07-08 [PMID: 27388773] (WB)

Bioinformatics

• Gene Symbol: UCHL1
• Entrez:
  • 7345(Human)
  • 22223(Mouse)
  • 29545(Rat)
• Uniprot:
  • P09936(Human)