UCH-L1/PGP9.5 Antibody (JF93-08)

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (JF93-08) [NBP2-66760] - Staining PGP9.5 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (JF93-08) [NBP2-66760] - Analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (JF93-08) [NBP2-66760] - Staining PGP9.5 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (JF93-08) [NBP2-66760] - Staining PGP9.5 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (JF93-08) [NBP2-66760] - Analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.

Product Discontinued

UCH-L1/PGP9.5 Antibody (JF93-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human UCH-L1/PGP9.5 aa 50-90 / 223. (SwissProt: P09936 Human; SwissProt: Q9R0P9 Mouse; SwissProt: Q00981 Rat)
• Localization: Cytoplasm, Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UCHL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UCH-L1/PGP9.5 Antibody (JF93-08)

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCH-L1/PGP9.5 Antibody (NBP2-66760)(1)

Publication using NBP2-66760

• Xia X, Xu Q, Liu M et al. Deubiquitination of CD36 by UCHL1 promotes foam cell formation Cell Death Dis 2020-08-15 [PMID: 32801299] (IP)

Bioinformatics

• Gene Symbol: UCHL1