UCH-L1/PGP9.5 Antibody (10A1)

Images

 
Immunohistochemistry: PGP9.5 / UCHL-1 Antibody (10A1) [NB100-1926] - PGP9.5 immunoreactivity in the cytoplasm of neuronal cell bodies in tracheal ganglion.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clone
10A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

UCH-L1/PGP9.5 Antibody (10A1) Summary

Immunogen
Recombinant full length protein (Human).
Localization
Cytoplasmic
Marker
pan-Neuronal Marker
Specificity
PGP9.5 (10A1)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 -1:1000
  • Immunohistochemistry 1:20-1:40
  • Immunohistochemistry-Paraffin 1:20-1:40
Positive Control
UCH-L1/PGP9.5 Lysate (NBL1-17575)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tissue culture supernatant
Preservative
15mM Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Tissue culture supernatant

Alternate Names for UCH-L1/PGP9.5 Antibody (10A1)

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

PGP9.5 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. It is present in neurons and in cells of the diffuse neuroendocrine system. PGP9.5 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. This enzyme is also known as UCH-L1. Because of its abundance in nerves, it has been widely used as a marker for peripheral nerve fibers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UCHL1
Entrez