UCH-L1/PGP9.5 Antibody (10A1)

Immunohistochemistry: PGP9.5 / UCHL-1 Antibody (10A1) [NB100-1926] - PGP9.5 immunoreactivity in the cytoplasm of neuronal cell bodies in tracheal ganglion.

Product Discontinued

UCH-L1/PGP9.5 Antibody (10A1) Summary

• Immunogen: Recombinant full length protein (Human).
• Localization: Cytoplasmic
• Marker: pan-Neuronal Marker
• Specificity: PGP9.5 (10A1)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: UCHL1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:20-1:40
  • Immunohistochemistry-Paraffin 1:20-1:40
  • Western Blot 1:500 -1:1000
• Application Notes: This UCH-L1/PGP9.5 Antibody (10A1) is validated for ICC/IF from a verified customer review.
• Control:

  UCH-L1/PGP9.5 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Alternate Names for UCH-L1/PGP9.5 Antibody (10A1)

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

PGP9.5 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. It is present in neurons and in cells of the diffuse neuroendocrine system. PGP9.5 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. This enzyme is also known as UCH-L1. Because of its abundance in nerves, it has been widely used as a marker for peripheral nerve fibers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for UCH-L1/PGP9.5 Antibody (NB100-1926) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 08/13/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: HeLa cells
• Species: Human
• Lot: 10A1

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: UCHL1
• Entrez:
  • 7345(Human)
  • 22223(Mouse)
  • 29545(Rat)