Ubiquitin B Antibody (83456)

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 83456
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Ubiquitin B Antibody (83456) Summary

• Immunogen: E. coli-derived recombinant human Ubiquitin+1
  Met1-Gly75, YADLREDPDRQDHHPGSGAQ (C-terminal sequence generated by a frameshift)
  Accession # CAA44911
• Specificity: Detects human Ubiquitin+1 in ELISAs and Western blots. In in ELISAs and Western blots, no cross-reactivity with Ubiquitin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human Ubiquitin+1 Biotinylated Antibody (Catalog number BAM7032)
  Standard: Recombinant Human Ubiquitin+1 (Catalog number 703-UB)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ubiquitin B Antibody (83456)

• FLJ25987
• MGC8385
• polyubiquitin B
• polyubiquitin-B
• RPS27A
• UBA52
• UBC
• ubiquitin B
• Ubiquitin+1

Background

Ubiquitin+1 has a carboxyl terminal amino acid sequence that differs from normal Ubiquitin. The different carboxyl terminal sequence appears to result from a frameshift in the Ubiquitin mRNA. The underlying mechanisms creating the mRNA frameshift are not clearly understood. The occurrence of the frameshift that generates Ubiquitin+1 is much more prevalent in patients with Alzheimers Disease or with Down Syndrome than in control individuals who are not afflicted with the disorders. The monoclonal anti-Ubiquitin+1 (Catalog # MAB703) and rabbit polyclonal anti-Ubiquitin+1 (Catalog # AF703) antibodies were raised against the Ubiquitin+1 carboxyl terminal sequence that differs from normal Ubiquitin and are therefore non-reactive with Ubiquitin. Monoclonal anti-Ubiquitin (Catalog # MAB701) detects both Ubiquitin and Ubiquitin+1 indicating that the epitope recognized by this antibody is contained in the portion of the proteins that are identical (1, 2).

1. van Leeuwen, F.W. et al. (1998) Science 279:242.
2. van Leeuwen, F.W. et al. (1998) Trends Neurosci. 21:331.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBB
• Uniprot:
  • CAA44911()