Ubiquitin B Antibody (83456) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clone: 83456
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

Ubiquitin B Antibody (83456) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human Ubiquitin+1 in ELISAs and Western blots. In in ELISAs and Western blots, no cross-reactivity with Ubiquitin is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ubiquitin B Antibody (83456) [Alexa Fluor™ Plus 680]

• FLJ25987
• MGC8385
• polyubiquitin B
• polyubiquitin-B
• RPS27A
• UBA52
• UBC
• ubiquitin B
• Ubiquitin+1

Background

Ubiquitin+1 has a carboxyl terminal amino acid sequence that differs from normal Ubiquitin. The different carboxyl terminal sequence appears to result from a frameshift in the Ubiquitin mRNA. The underlying mechanisms creating the mRNA frameshift are not clearly understood. The occurrence of the frameshift that generates Ubiquitin+1 is much more prevalent in patients with Alzheimers Disease or with Down Syndrome than in control individuals who are not afflicted with the disorders. The monoclonal anti-Ubiquitin+1 (Catalog # MAB703) and rabbit polyclonal anti-Ubiquitin+1 (Catalog # AF703) antibodies were raised against the Ubiquitin+1 carboxyl terminal sequence that differs from normal Ubiquitin and are therefore non-reactive with Ubiquitin. Monoclonal anti-Ubiquitin (Catalog # MAB701) detects both Ubiquitin and Ubiquitin+1 indicating that the epitope recognized by this antibody is contained in the portion of the proteins that are identical (1, 2).

1. van Leeuwen, F.W. et al. (1998) Science 279:242.
2. van Leeuwen, F.W. et al. (1998) Trends Neurosci. 21:331.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.