Ubiquitin B Antibody (3C12) - BSA Free

Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.

Flow Cytometry: Ubiquitin B Antibody (3C12) [NBP2-37521] - Flow cytometric analysis of Hela cells using UBB mouse mAb (green) and negative control (red).

Western Blot: Ubiquitin B Antibody (3C12) [NBP2-37521] - Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombinant protein. (Expected MW is 26 kDa)

ELISA: Ubiquitin B Antibody (3C12) [NBP2-37521] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 3C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Ubiquitin B Antibody (3C12) - BSA Free Summary

• Description: Novus Biologicals Mouse Ubiquitin B Antibody (3C12) - BSA Free (NBP2-37521) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Ubiquitin B expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Ubiquitin B Antibody (3C12) - BSA Free

• FLJ25987
• MGC8385
• polyubiquitin B
• polyubiquitin-B
• RPS27A
• UBA52
• UBC
• ubiquitin B
• Ubiquitin+1

Background

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBB