UBE2M/Ubc12 Antibody - BSA Free

Western Blot: UBE2M/Ubc12 Antibody [NBP1-31459] - Sample(30 ug whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: UBE2M/Ubc12 Antibody [NBP1-31459] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, Rt, Bv, RM, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBE2M/Ubc12 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2M/Ubc12. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Rat (100%), Xenopus (95%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2M
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for UBE2M/Ubc12 Antibody - BSA Free

• EC 6.3.2
• EC 6.3.2.-
• hUbc12
• NEDD8 carrier protein
• NEDD8 protein ligase
• UBC12
• UBC12NEDD8-conjugating enzyme Ubc12
• UbcH12
• UBC-RS2
• UBE2M
• ubiquitin carrier protein M
• Ubiquitin-conjugating enzyme E2 M
• ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
• ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• ubiquitin-protein ligase M
• yeast UBC12 homolog

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for UBE2M/Ubc12 Antibody (NBP1-31459)(1)

Publication using NBP1-31459

• Vava A, Paccez JD, Wang Y et al. DCUN1D1 Is an Essential Regulator of Prostate Cancer Proliferation and Tumour Growth That Acts through Neddylation of Cullin 1, 3, 4A and 5 and Deregulation of Wnt/Catenin Pathway Cells 2023-07-31 [PMID: 37566052] (Western Blot)

Bioinformatics

• Gene Symbol: UBE2M