UBE2M/Ubc12 Antibody

Images

 
Western Blot: UBE2M/Ubc12 Antibody [NBP1-31459] - Sample(30 ug whole cell lysate) A:293T 12% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: UBE2M/Ubc12 Antibody [NBP1-31459] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, XpSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.51 mg/ml

Order Details

UBE2M/Ubc12 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2M. The exact sequence is proprietary.
Predicted Species
Mouse (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (95%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2M
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.51 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE2M/Ubc12 Antibody

  • EC 6.3.2
  • EC 6.3.2.-
  • hUbc12
  • NEDD8 carrier protein
  • NEDD8 protein ligase
  • UBC12
  • UBC12NEDD8-conjugating enzyme Ubc12
  • UbcH12
  • UBC-RS2
  • UBE2M
  • ubiquitin carrier protein M
  • Ubiquitin-conjugating enzyme E2 M
  • ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12)
  • ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
  • ubiquitin-protein ligase M
  • yeast UBC12 homolog

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Diseases for UBE2M/Ubc12 Antibody (NBP1-31459)

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Bioinformatics

Gene Symbol UBE2M
Entrez