TXNDC5 Antibody (2E7/7) - BSA Free

Immunocytochemistry/Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 2E7/7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TXNDC5 Antibody (2E7/7) - BSA Free Summary

• Description: Novus Biologicals Mouse TXNDC5 Antibody (2E7/7) - BSA Free (NBP2-22414) is a monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
• Localization: Endoplasmic Reticulum , Endoplasmic Reticulum Lumen
• Specificity: Detects approx 48kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TXNDC5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:200
• Application Notes: 1 ug/ml of EndoPDI Antibody was sufficient for detection of EndoPDI in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TXNDC5 Antibody (2E7/7) - BSA Free

• EndoPDI
• ERp46
• FLJ90810
• HCC-2
• member 15
• MGC3178
• PDIA15
• thioredoxin domain containing 5 (endoplasmic reticulum)
• thioredoxin domain containing 5
• thioredoxin domain-containing protein 5
• thioredoxin related protein
• TLP46
• TXNDC5

Background

TXNDC5 encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. This gene can be co-transcribed with the upstream gene MU.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXNDC5
• Uniprot:
  • Q8NBS9(Human)