Twist-2 Antibody (OTI6H10) - Azide and BSA Free

Western Blot: Twist-2 Antibody (OTI6H10) - Azide and BSA Free [NBP2-74724] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWIST2 .

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI6H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Twist-2 Antibody (OTI6H10) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human TWIST2 (NP_476527) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TWIST2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 17.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Twist-2 Antibody (OTI6H10) - Azide and BSA Free

• bHLHa39
• bHLHa39twist-related bHLH protein Dermo1
• Class A basic helix-loop-helix protein 39
• Dermis-expressed protein 1
• DERMO1
• Dermo-1
• DERMO1MGC117334
• twist homolog 2 (Drosophila)
• Twist2
• Twist-2
• twist-related protein 2

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TWIST2